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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 204-371-1 | CAS number: 120-12-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Acute lethal toxicity of hydrocarbons and chlorinated hydrocarbons to two planktonic crustaceans: the key role of organism-water partitioning
- Author:
- Abernethy S, Bobra AM, Shiu WY, Wells PG, Mackay D
- Year:
- 1 986
- Bibliographic source:
- Aquat. Toxicol. 8, 163-174 (1986)
- Reference Type:
- publication
- Title:
- A predictive correlation for the acute toxicity of hydrocarbons and chlorinated hydrocarbons to the water flea (Daphnia magna)
- Author:
- Bobra AM, Shiu WY, Mackay D
- Year:
- 1 983
- Bibliographic source:
- Chemosphere 12, 1121-1129 (1983)
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Age of daphnia: 4 - 6 days
- Principles of method if other than guideline:
- Method: Acute Daphnia study, static, closed system, without UV light
- GLP compliance:
- no
Test material
- Reference substance name:
- Anthracene
- EC Number:
- 204-371-1
- EC Name:
- Anthracene
- Cas Number:
- 120-12-7
- Molecular formula:
- C14H10
- IUPAC Name:
- anthracene
- Details on test material:
- - Sorurce: commercial
- Purity >= 97 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Aqueous saturated stock solution in distilled water, obtained by vigorous stirring (magnetic stirrer)
with excess test material under sealed conditions. Afterwards, 48 h settling before start of the bioassay.
- Differential loading: no, dilutions from stock solution
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Laboratory culture of parthenogenetic females
- Age at study initiation (mean and range, SD): 4 - 6 days
- Length at study initiation (length definition, mean, range and SD): mean 1.5 mm
- Method of breeding: Stock culture held in distilled water at about pH 6.5
- Feeding during test: no
- Food type: trout chow and algae
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- under sealed conditions
Test conditions
- Hardness:
- --
- Test temperature:
- 23 +-2 °C
- pH:
- --
- Dissolved oxygen:
- >= 5 mg/L
- Salinity:
- --
- Nominal and measured concentrations:
- no data: 5 test concentrations
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 33 mL, no headspace
- Aeration: no
- Renewal rate of test solution: no
- No. of organisms per vessel: 10 (Bobra et al 1983)
- No. of vessels per concentration (replicates): 2 -4 (Bobra et al 1983)
- No. of vessels per control (replicates): same as tests
- No. of vessels per vehicle control (replicates): same as tests
- Biomass loading rate: no data
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: distilled
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality - Reference substance (positive control):
- yes
- Remarks:
- Phenol
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 36 µg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: LC50 = 0.5 of the saturated solution; without UV
- Details on results:
- LC50 was not measured or derived from a definite nominal concentration, but derived from the known (prior determined) maximum water solubility (saturated solution). Water saturation was assumed to be 0.4 µmol/L = 72 µg/L (Data from Mackay, D.; Shiu, W.Y. 1981: A critical review of Henry´s Law Constants for chemicals of environmental interest. J. Phys. Chem. Ref. Data 10, 1175-1199).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- O2 content >=5 mg/L; mortality in controls =< 10 %
- Conclusions:
- The LC50 was found to be within the range of water solubility without the influence of UV light.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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