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EC number: 204-385-8 | CAS number: 120-32-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
NOEC (30 d) < 0.0095 mg test item/L (Mortality, OECD 210, nominal, Schneider, Wydra and Böttcher, 2007)
NOEC (30 d): 0.31 mg test item/L (Hatching success, OECD 210, nominal, Schneider, Wydra and Böttcher, 2007)
NOEC (30 d): ≥ 0.031 mg test item/L (Growth parameters, OECD 210, nominal, Schneider, Wydra and Böttcher, 2007)
NOEC (30 d): 0.58 µg test item/L (Mortality, OECD 210, mean measured, Böttcher and Wydra 2008)
NOEC (30 d): 73.0 µg test item/L (Hatching success, OECD 210, mean measured, Böttcher and Wydra 2008)
NOEC (30 d): 22.85 µg test item/L (Growth parameters, OECD 210, mean measured, Böttcher and Wydra 2008)
Key value for chemical safety assessment
Additional information
Two experimental studies are available investigating the effects of the test item Chlorophene (CAS 120-32-1) on fish early life stages of the freshwater species Danio rerio (Schneider, Wydra & Böttcher, 2007 and Böttcher and Wydra, 2008). The studies were conducted according to GLP and OECD guideline 210 (2013).
For the first study (Schneider, Wydra & Böttcher, 2007) 60 fertilised eggs each were exposed under flow-through conditions to nominal concentrations of 0.0095, 0.031, 0.098, 0.31 and 1.0 mg chlorophene/L (mean measured concentrations ranged from 78% to 100% of nominal) and a control until 30 days after hatching of the larvae. The daily recorded effects were mortality, hatching success and symptoms of intoxication. Furthermore, at the start and the end of the test, the growth parameters body weight and length of surviving fish were determined. The resulting NOEC based on mortality was < 0.0095 mg test item/L, the lowest concentration tested. The LOEC was determined to be 0.0095 mg test item/L. NOEC based on hatching success was 0.31 mg test item/L and based on growth parameters ≥ 0.031 mg test item/L. An exact NOEC could not be determined as the lowest concentration tested showed significantly different effects from control. Thus a NOEC of < 0.0095 mg Chlorophene/L obtained for mortality is reported.
For the second study the fertilized eggs of Zebra-fish (Danio rerio) were exposed in a flow-through test to aqueous test media containing the test item at concentrations of nominal 100, 31.3, 9.77, 3.05, 0.95 µg/L and to a deionised water control under defined conditions. The test duration was 30 days after hatching. The recorded effects were mortality, hatching, growth and symptoms of intoxication of the fish. Mean recovery rates of 54 to 81% were measured in the test concentrations. Therefore, analytically determined mean concentrations were used for the calculation and reporting of results. The concentration of the test item in the test media of nominal 0.95 µg/L was slightly below the Limit of Quantification. However, the data were considered reliable. The LC50 was determined to be 1.10 µg test item/L (mean measured). The validity criteria can be considered as fulfilled. The NOEC for hatching success was 73.00 µg test item/L (mean measured) and ≥ 100 µg test item/L (nominal)The NOEC for Mortality/survival (30 d post-hatch) was ≥ 0.58 µg test item/L (mean measured) and ≥ 0.95 µg test item/L (nominal). The NOEC for body length was ≥ 22.85 µg test item/L (mean measured) and ≥ 31.3 µg test item/L (nominal). The NOEC for body weight was ≥ 22.85 µg test item/L (mean measured) and ≥ 31.3 µg test item/L (nominal). For Zebra-fish, the highest concentration tested without toxic effects (NOEC) of the test item Chlorophene on mortality after an exposure period of 30 days was determined to be 0.58 µg test item/L (mean measured). The lowest concentration tested with toxic effects (LOEC) on mortality was determined to be 1.65 µg test item/L (mean measured). The LC50 value for mortality has been calculated to be 1.10 µg test item/L (mean measured).
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