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EC number: 204-798-3 | CAS number: 126-71-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- analytical verification of the test substance is missing, not performed according to GLP and OECD guidelines,
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412, Part 11
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- Cremophor
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Bundesgesundheitsamt Berlin, Germany
- Method of breeding: single breeding in 100 mL beaker - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 21 °C
- pH:
- 7.2 - 7.6
- Dissolved oxygen:
- 8.1 - 8.4 mg/L
- Nominal and measured concentrations:
- Test concentrations (nominal): 0.58, 1.0, 1.8, 3.2, 5.8, 10, 18, 32, 58 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass beaker
- Fill volume: 20 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412, Part 11
- Ca/mg ratio: 5/1
- Conductivity: 658 µS/cm - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 5.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL 9 - 12 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50 (24h): 1.1 mg/L potassium dichromate
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 18200055
- Expiration date of the lot/batch: 30 June 2020
- Purity: 98.3%
- Analytical monitoring:
- yes
- Details on sampling:
- Each concentration and the control were analytically verified in the fresh media at the start of exposure solution (0 hours) as well as in the aged at the end of the exposure (48 hours). At the start of the exposure (0 hours), three samples per concentration level and control were taken after preparation of all concentration levels and analyzed. At the end of the exposure (48 hours), three samples of the 48-hours old media per concentration level and control were taken directly from the test vessels.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared by adding an appropriate amount of the test item (100 mg/L) to dilution water. The stock solution and a further 4 test item concentrations were prepared by diluting the stock solution accordingly.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: 5
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 +- 2 °C, in an incubator, 16 hours illumination, light intensity of max. 1500 lx
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), Berlin, Germany
- Feeding during test : none
ACCLIMATION
- Acclimation period: continuous culturing at the test facility
- Acclimation conditions (same as test or not): same
- Type and amount of food: mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus, ad libitum
- Feeding frequency: 5 times per week - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 18 – 22 °C
- Nominal and measured concentrations:
- Nominal: 6.25, 12.5, 25.0, 50.0 and 100 mg/L
Measured (test inition): 6.47, 13.1, 27.2, 50.9 and 108 mg/L
Measured (48h): 5.93, 11.3, 23.3, 45.3 and 92.6 mg/L
Measured > 80% nominal - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity, loosely covered with watch glasses
- Volume of solution: 20 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: At the start of the exposure (0 hours), the water quality parameters of the fresh media (i.e. pH-value, dissolved oxygen concentration) were measured in one additional replicate (without daphnids) per concentration level and control. At the end of the exposure (48 hours), the water quality parameters of the old media were measured in one appropriate replicate (containing daphnids) per concentration level and control. The replicate with the highest immobilization rate per concentration level and control was measured.
OTHER TEST CONDITIONS
- Photoperiod: 16h light
- Light intensity: Diffuse light, light intensity of max. 1500 lx
EFFECT PARAMETERS MEASURED : Immobility was recorded after 24 and 48h exposure time.
- Adjustment of pH:
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
VEHICLE CONTROL PERFORMED: yes/no
RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: analytically verified
- Details on results:
- - Behavioural abnormalities:
none
- Other biological observations: none
- Mortality of control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no - Results with reference substance (positive control):
- - Results with reference substance valid?
yes
- Relevant effect levels: 24h-EC50 = 2.03 mg/L
- validity range: 0.6 - 2.4 mg/L, acc. to AQS P 9/2 (02/2000); clone 5: - Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance showed a 48h-EC50 of 24 mg/L and thus, to be harmful to aquatic invertebrates.
Referenceopen allclose all
Description of key information
EC50(48h) = 24 mg/L (nominal, analytically verified) for immobilisation of Daphnia magna (OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 24 mg/L
Additional information
Two studies are available investigating the short-term toxicity of triisubutyl phosphate to aquatic invertebrates. However, one of the studies (BASF 1989) was considered to be not reliable due to methodological shortcomings (missing analytical verification of test concentrations, not performed according to GLP and current OECD guidelines). Thus, a new test was performed according to current quality criteria. This key study was performed according to GLP and OECD giudeline 202 using Daphnia magna as test organism (Herrmann 2019). Analytical verification of the test concentrations at the beginning and the end of the test showed > 80% of nominal at any measurement. No mortality was observed in the control. The 48h-EC50 is determined to be 24 mg/L based on nominal concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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