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EC number: 213-651-2 | CAS number: 998-40-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-07-05 - 2017-11-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to GLP in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Also used for preparation of the test substance: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, 2000.
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, 2000
Preparation of test solutions started with a loading rate of 100 mg/L applying a one day period of magnetic stirring to ensure maximum dissolution of the test item in medium. The obtained mixture was allowed to settle for 13 minutes. Thereafter, the aqueous Saturated Solution (SS) was collected by means of siphoning and used as the highest test concentration. Lower test concentrations were prepared by subsequent dilutions of the SS in test medium. All test solutions were clear and colorless at the end of the preparation procedure. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna (Crustacea, Cladocera) (Straus, 1820), at least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions.
- Source: Charles River Laboratories, In-house laboratory culture with a known history
- Age of parental stock (mean and range, SD): Maximum age of the cultures 4 weeks
- Feeding during test: No feeding - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 18-22 °C, constant within 2 °C
- pH:
- pH 7.7-7.9
- Dissolved oxygen:
- 8.9-7.8 mg/L
- Nominal and measured concentrations:
- 0.46%, 1.0%, 2.2%, 4.6%, 10% and 22% of a SS prepared at a loading rate of 100 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 60 mL, all-glass, airtight closed and completely filled with no headspace
- Type: closed
- Aeration: no aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- Potassium dichromate 48h-EC50 0.59 mg/L, within the ranges of the expected responses at the different concentrations, i.e. the 48h-EC50 was between 0.3 and 1.0 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, the 48h-EC50 for Daphnia magna exposed to Tri-n-butyl phosphine equalled a concentration representing 9.2% of a saturated solution prepared at a loading rate of
100 mg/L (95% confidence interval between 7.5% and 11% SS). - Executive summary:
The acute toxicity of the test material to freshwater invertebrates was determined in a study conducted in accordance with the standardised guideline OECD 202 under GLP conditions. Daphnia magna were exposed to the test material for 48 hours under static conditions at nominal concentrations 0.46%, 1.0%, 2.2%, 4.6%, 10% and 22% of a SS prepared at a loading rate of 100 mg/L (no solvent). A SS prepared with loading rate was used due to the poor solubility of the test substance.
Under the conditions of the test, the 48h EC50 of the test material to Daphnia magna was determined to be 9.2% of a SS prepared at a loading rate of 100 mg/L.
Reference
Table 1 - Number of Introduced Daphnids and Incidence of
Immobility in the Range-Finding Test
Time (h) |
Replicate |
Tri-n-butyl phosphine (% SS prep. at 100 mg/L) |
||||
Control |
0.10 |
1.0 |
10 |
|
||
0 |
A |
5 |
5 |
5 |
5 |
|
B |
5 |
5 |
5 |
5 |
|
|
Total introduced |
10 |
10 |
10 |
10 |
|
|
|
||||||
24 |
A |
0 (1) |
0 |
0 |
0 |
|
B |
0 |
0 |
0 |
0 (1) |
|
|
Total immobilised |
0 |
0 |
0 |
0 |
|
|
Effect % |
0 |
0 |
0 |
0 |
|
|
|
|
|
|
|
||
48 |
A |
0 (1) |
0 |
0 |
2 |
|
B |
0 (4) |
0 |
0 |
2# |
|
|
Total immobilised |
0 |
0 |
0 |
4 |
|
|
Effect % |
0 |
0 |
0 |
40 |
|
( ) between brackets: number of daphnia observed trapped at the surface of the test solutions. These organisms were reimmersed into the respective solutions before recording of mobility
# Microscopic observation revealed no test item attached to the daphnids.
Table 2 - Number of Introduced Daphnids and Incidence of
Immobility in the Final Test
Time (h) |
Replicate |
Tri-n-butyl phosphine (% SS prep. at 100 mg/L) |
|
|||||
Control |
0.46 |
1.0 |
2.2 |
4.6 |
10 |
22 |
||
0 |
A |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
B |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
C |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
D |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
Total introduced |
20 |
20 |
20 |
20 |
20 |
20 |
20 |
|
|
||||||||
24 |
A |
0 |
0 |
0 |
0 |
0 |
0 |
2 (1) |
B |
0 |
0 |
0 |
0 |
0 |
0 |
0 (2) |
|
C |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
|
D |
0 |
0 |
0 |
0 |
0 |
0 |
1 (1) |
|
Total immobilised |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
|
Effect % |
0 |
0 |
0 |
0 |
0 |
0 |
25 |
|
|
|
|
|
|
|
|
||
48 |
A |
0 |
0 |
0 |
0 |
0 |
4# |
5 |
B |
0 |
0 |
0 |
0 |
1* |
3 |
5 (3) |
|
C |
0 (3) |
0 |
0 |
0 |
0 |
2 |
5 |
|
D |
0 |
0 |
0 |
0 |
0 |
2 |
5 |
|
Total immobilised |
0 |
0 |
0 |
0 |
1 |
11 |
20 |
|
Effect % |
0 |
0 |
0 |
0 |
5 |
55 |
100 |
( ) between brackets: number of daphnia observed trapped at the surface of the test solutions. These organisms were reimmersed into the respective solutions before recording of mobility
# Microscopic observation revealed no test item attached to the daphnids
* A total of 6 daphnids were observed (seeAppendix 3).
Description of key information
Under the conditions of the test, the 48h EC50 of the test material to Daphnia magna was determined to be 9.2% of a SS prepared at a loading rate of 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 9.2 mg/L
Additional information
The acute toxicity of the test material to freshwater invertebrates was determined in a study conducted in accordance with the standardised guideline OECD 202 under GLP conditions. Daphnia magna were exposed to the test material for 48 hours under static conditions at nominal concentrations 0.46%, 1.0%, 2.2%, 4.6%, 10% and 22% of a SS prepared at a loading rate of 100 mg/L (no solvent). A SS prepared with loading rate was used due to the poor solubility of the test substance.
Under the conditions of the test, the 48h EC50 of the test material to Daphnia magna was determined to be 9.2% of a SS prepared at a loading rate of 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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