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EC number: 224-867-1 | CAS number: 4531-49-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-11-08 - 1993-12-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- GLP according to directive 88/320 EEC, date of inspection: 1992-03-17
Test material
- Reference substance name:
- 2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(4-methoxyphenyl)-3-oxobutyramide]
- EC Number:
- 250-797-6
- EC Name:
- 2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(4-methoxyphenyl)-3-oxobutyramide]
- Cas Number:
- 31775-16-3
- Molecular formula:
- C34H30Cl2N6O6
- IUPAC Name:
- 2,2'-[(3,3'-dichlorobiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis[N-(4-methoxyphenyl)-3-oxobutanamide]
- Test material form:
- not specified
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Type: A mixed population of activated sludge microorganisms
Source: The aeration stage of the Severn Trent Water Plc sewage treatment plant at Belper, Derbyshire, treating predominantly domestic sewage.
Date of collection: 8 November 1993.
Pre-Treatment: The activated sludge was homogenised for approximately 2 minutes and allowed to settle for 1/2 hour. The supernatant was centrifuged to remove coarse solids and used for testing.
Usage rate: 1% inoculum. - Duration of test (contact time):
- 28
Initial test substance concentration
- Initial conc.:
- 33.8 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Test sample:
Method of Preparation: Direct dispersion of the test substance in culture medium
Exposure
Duration: 28 days.
Vessels: 5 litre glass culture vessels containing 3 litres.
Culture medium: As specified in OECD-guideline 301
Loading: 1% inoculum per test vessel.
Test concentration: 20 mg carbon/L in duplicate.
Reference material 10 mg carbon/L sodium benzoate, in duplicate.
Test series:
1. Culture medium with inoculum in duplicate.
2. Culture medium with inoculum and sodium benzoate in duplicate
3. Culture medium with inoculum and test substance in duplicate.
4. Culture medium with inoculum and test substance, poisoned by the addition of 10 ml of a 10 g/1 mercuric chloride solution to act as an abiotic control (one vessel only).
Temperature: 21 ± 1°C
Light regime: The study was carried out in darkness.
Procedure: Approximately 24 hours prior to the start of the study the vessels were filled with 2400 mL of culture medium and 30 mL of inoculum and aerated overnight. On day 0 the test and standard materials were added and the volume in all vessels made up to 3 litres by the addition of culture medium. The CO2 absorption bottles were connected to the outlet of the vessels on day 0.
Aeration: The culture vessels were sealed and CO2-free air bubbled through the solution at a rate of approximately 40 mL/min and stirred continuously by magnetic stirrers. CO2-free air was produced by sparging compressed air through the following series:
- 3 x 500 ml Dreschel bottles filled with 350 mL 10 N NaOH.
- 1 x 500 ml Dreschel bottle filled with 350 mL 0.025 N Ba(OH)2
- 1 x 500 ml empty Dreschel bottle to prevent liquid carry-over.
CO2-absorption: 2 x 500 mL Dreschel bottles filled with 350 mL 0.05 M NaOH. All CO2 absorbing solutions were prepared using purified de-gassed water.
Sampling: Samples (2 mL) were taken from the first CO2 absorber vessel on days 0, 1, 2, 3, 6, 8, 10, 12, 14, 16, 20, 22, 24, 27, 28 and 29. The second absorber vessel was sampled on days 0 and 29.
On day 28 the pH of each test vessel was measured and 1 mL of concentrated HCl added to drive off inorganic carbonate. The vessels were aerated overnight and the final samples were taken from both absorber vessels on day 29.
Apparatus: Ionics 555 TOC Anlyser. Each analysis was carried out in triplicate.
Evaluation: All calculations were performed according to the guideline (OECD 301B).
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- not applicable
- Test performance:
- not applicable
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 28 d
- Details on results:
- The pH values of the test material, standard material and control cultures an day 28 were 7.5 and 7.5 (test material), 7.5 and 7.5 (standard material), 7.5 and 7.5 (control) and 7.4 (abiotic control).
The test substance attained 1% degradation after 28 days and so, therefore, cannot be considered as readily biodegradable under the strict terms and conditions of the OECD Guidelines.
Additional investigational work was carried out using the Activated Sludge Respiration Inhibition test method (OECD Guideline No. 209) which showed that the test substance did not inhibit the respiration of sewage sludge microorganisms at the test concentration employed in the test. In the light of this the inhibition control vessel (test substance plus sodium benzoate) was omitted from the test.
Therefore from these results it is evident that the test substance is not readily biodegradable and that this result is not due to a toxic effect on the inoculum.
BOD5 / COD results
- Results with reference substance:
- Sodium benzoate attained 86% degradation after 28 days thereby confirming the suitability of the inoculum and test conditions.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- A ready biodegradability test on the test substance according to OECD 301B (CO2 -evolution) and further testing for activated sludge respiration inhibition (OECD 209, results only mentioned in the study report) it is evident that the test substance is not readily biodegradable (1% biodegradation) and that this result is not due to a toxic effect on the inoculum.
Sodium benzoate attained 86% degradation after 28 days thereby confirming the suitability of the inoculum and test conditions. - Executive summary:
In a ready biodegradablility test (reliability categoriy 1) on the test substance according to OECD 301B (CO2 -evolution) and complient to GLP, the test substance attained 1% degradation at a concentration of 20 mg C/L. To exclude toxicity of the test item to the inoculum at the tested concentration additional investigational work was carried out using the Activated Sludge Respiration Inhibition test method (OECD Guideline No. 209, results mentioned in this report, but no study report available) which showed that the test substance did not inhibit the respiration of sewage sludge microorganisms at the test concentration employed in the test. Therefore from these results it is evident that the test substance is not readily biodegradable and that this result is not due to a toxic effect on the inoculum.
Sodium benzoate attained 86% degradation after 28 days thereby confirming the suitability of the inoculum and test conditions.
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