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EC number: 226-685-8 | CAS number: 5451-76-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-butoxyethyl benzoate
- EC Number:
- 226-685-8
- EC Name:
- 2-butoxyethyl benzoate
- Cas Number:
- 5451-76-3
- Molecular formula:
- C13H18O3
- IUPAC Name:
- 2-butoxyethyl benzoate
- Test material form:
- other: liquid
- Details on test material:
- 2-Butoxyethyl benzoate, Lot # 201303443-19; Purity 99.2 %
Constituent 1
- Specific details on test material used for the study:
- Test Material Name: 2-Butoxyethyl benzoate
Chemical Name: 2-Butoxyethanol benzoate
Supplier, City, State (Lot, Reference Number): The Dow Chemical Company, Midland, Michigan (Lot # 201303443-19).
Purity/Characterization (Method of Analysis and Reference): The purity of the test material was determined to be 99.2% area (corrected for water) by gas chromatography with identification by nuclear magnetic resonance and gas chromatography mass spectrometry (Gobbi, 2014).
Test Material Stability Under Storage Conditions: The test material was determined to have two years of stability under ambient storage conditions (Wachowicz et al., 2015).
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge was obtained from the Midland Municipal Wastewater Treatment Plant
(Midland, Michigan). This facility treats an excess of 3.0 x 106 gallons of wastewater
daily, of which > 90% is from domestic sources. Upon return to the laboratory, the
activated sludge was washed three times with tap water.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
Test conditions
- Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
Any other information on results incl. tables
Initial Reaction Mixtures Rate (mg/L/hr) % Inhibition
Control (C1) 12.108 NA
Reference Mixture 1 (3 mg/L) 11.154 14.71
Reference Mixture 2 (10 mg/L) 9.072 30.63
Reference Mixture 3 (30 mg/L) 3.6 72.47
Test Mixture 1 (100 mg/L) 13.74 -5.07
Test Mixture 2 (100 mg/L) 11.67 10.76
Test Mixture 3 (300 mg/L) 9.294 28.93
Test Mixture 4 (300 mg/L) 9.27 29.11
Test Mixture 5 (1000 mg/L) 11.226 14.15
Test Mixture 6 (1000 mg/L) 10.968 16.13
Control (C2) 14.046 NA
Three hour reaction mixtures
Control (C1) 11.916 NA
Reference Mixture 1 (3 mg/L) 10.494 16.65
Reference Mixture 2 (10 mg/L) 7.548 40.05
Reference Mixture 3 (30 mg/L) 3.24 74.27
Test Mixture 1 (100 mg/L) 15.708 -24.76
Test Mixture 2 (100 mg/L) 16.518 -31.19
Test Mixture 3 (300 mg/L) 13.638 -8.32
Test Mixture 4 (300 mg/L) 13.614 -8.12
Test Mixture 5 (1000 mg/L) 10.704 14.99
Test Mixture 6 (1000 mg/L) 10.77 14.46
Control (C2) 13.266 NA
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The potential for 2-butoxyethyl benzoate to inhibit the respiration of municipal activated sludge was evaluated using the OECD 209 “Activated Sludge, Respiration Inhibition Test.” This study assessed the potential effect of a range of test chemical concentrations on measured activated sludge respiration rates following both 30 min. and three hour contact periods. The 2-butoxyethyl benzoate substance, at nominal concentrations of 100, 300, and 1000 mg/L did not show > 50% inhibition of the activated sludge respiration rate after either 30 min. or three hours contact times. Therefore, the 30 min and three hour EC50 values were determined empirically to be EC50 > 1,000 mg/L. A statistical comparison (ANOVA) of respiration rates at each test material concentration and contact time resulted in determination of no observed effect concentrations (NOEC) for 30 min. and three hours contact times of 300 mg/L and 1,000 mg/L, respectively.
Considering that these EC50 and NOEC concentrations are well above the measured water solubility limit of the test material (i.e., 106 mg/L at 20 °C), the 2-butoxyethyl benzoate substance is expected to have very low potential for adversely affecting biological wastewater treatment operations. - Executive summary:
The potential for 2-butoxyethyl benzoate to inhibit the respiration of municipal activated sludge was evaluated using the OECD Guideline 209 “Activated Sludge, Respiration Inhibition Test.” This test method assesses the potential effect of a range of test chemical concentrations on measured activated sludge respiration rates following a three-hour contact period. In this study, the potential impact on respiration rates was also evaluated after a 30 min. contact period, in consideration of the ready biodegradability and volatile properties of the test material. The 2-butoxyethyl benzoate substance, at nominal concentrations of 100, 300, and 1000 mg/L did not show > 50% inhibition of the respiration rate of the activated sludge after either 30 min. or three hours contact time.
Therefore, the 30 min and three hour EC50 values were determined empirically to be EC50 > 1,000 mg/L. A statistical comparison (ANOVA) of respiration rates at each test material concentration and contact time resulted in determination of no observed effect concentrations (NOEC) for 30 min. and three hours contact times of 300 mg/L and 1,000 mg/L, respectively. Further testing of potential impact of the test material on nitrifying activity of activated sludge was deemed to be unnecessary, because respiration of nitrifying bacteria represents a relatively small proportion of total activated sludge respiration, and very high concentrations of test material (above solubility limit) were necessary to impact total respiration. Considering that these EC50 and NOEC concentrations are well above the measured water solubility limit of the test material (i.e., 106 mg/L at 20 °C), the 2-butoxyethyl benzoate substance is expected to have very low potential for adversely affecting biological wastewater treatment operations.
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