Ethyl 2-(dimethylamino)-1-phenylcyclohex-3-ene-1-carboxylate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study in compliance with GLP-criteria.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl trans-(±)-2-(dimethylamino)-1-phenylcyclohex-3-ene-1-carboxylate hydrochloride

EC Number:

248-226-0

EC Name:

Ethyl trans-(±)-2-(dimethylamino)-1-phenylcyclohex-3-ene-1-carboxylate hydrochloride

Cas Number:

27107-79-5

IUPAC Name:

27107-79-5

Details on test material:

- Substance name: Trans-Tilidin-HCl-Hemihydrate
- Aggregate state: solid, powder
- Homogeneity: homogeneous
- lnstability against: light, oxygen, water, alkali hygroscopic
- Storage conditions: at room temperature under nitrogen atmosphere and light exclusion.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge from laboratory wastewater plant treating municipal sewage. Concentration of dry substance 1 g/L. The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Test conditions

pH:

7.2-7.8 (after correction)

Dissolved oxygen:

7.4-6.2 mg/L

Nominal and measured concentrations:

- Nominal concentration: 1000 mg/L

Details on test conditions:

- Test vessels: Erlenmeyer-vessel (nominal volume 250 mL)
- Test volume: 250 mL
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium
- Oxygen concentration during aeration: >2.5 mg/L
- Oxygen concentration immediately before measurement: >6.5 mg/L
- Duration of the measurement of oxygen consumption: 8 - 10 min

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Results with reference substance (positive control):

EC20 = ca. 2.3 mg/L
EC50 = ca. 18 mg/L