Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 241-659-6 | CAS number: 17675-60-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July - August 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Chemical name: Amidinourea phosphate
- CAS No.: 75701-36-9
- Molecular formula: C2H6N4O.H3PO4
- Molecular weight: 200.1 g/mol - Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the different aqueous extracts were collected at time 0, 2 hours, and after 5 days.
- Buffers:
- CITRATE BUFFER (0.05 M): pH 4
23 g of potassium dihydrogen citrate were dissolved in 1 L of water. An aliquot of 0.50 L was mixed with 0.09 L of 0.1 M NaOH and filled up with water to 1 L to give a pH of 4. The pH was checked with a calibrated pH meter to be 4.07 at 20 °C.
PHOSPHATE BUFFER (0.05 M): pH 7
13.6 g of potassium dihydrogen phosphate were dissolved in 1 L of water. An aliquot of 0.50 L was mixed with 0.296 L of 0.1 M NaOH and filled up with water to 1 L to give a pH of 7. The pH was checked with a calibrated pH meter to be 7.10 at 20 °C.
BORATE BUFFER (0.05 M): pH 9
19 g of sodium tetraborate decahydrate were dissolved in 1 L of water. An aliquot of 0.85 L was mixed with 0.15 L of 0.1 M HCl (prepared from 10 M HCl, Chemsolute, 32 %) to give a pH of 9. The pH was checked with a calibrated pH meter to be 9.10 at 20 °C. - Estimation method (if used):
- none
- Details on test conditions:
- A stock solution of the test item was prepared by accurately weighing 200 mg of guanylurea phosphate into a 10 mL volumetric flask using an analytical balance and dissolving it in water (resulting concentration of analyte: 20.0 mg/mL). A fortification solution with 2 g/L (mg/mL) was prepared by dilution of the stock solution with Millipore water. Calibration solutions of Guanylurea Phosphate were prepared by volumetric dilution of the stock solution using milli-pore water as a solvent
- Duration:
- 5 d
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- >= 150 - <= 20 000 mg/L
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- >= 150 - <= 20 000 mg/L
- Duration:
- 5 d
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- >= 150 - <= 20 000 mg/L
- Number of replicates:
- Hydrolysis testing at each pH level was performed with three replicate test solutions and one blank for each buffer solution.
- Positive controls:
- yes
- Negative controls:
- yes
- Statistical methods:
- none
- Preliminary study:
- A preliminary hydrolysis experiment (Tier 1) was conducted as the hydrolytic behavior of the test substance was unknown so far. All three-buffer solution were spiked with test substance to achieve an end concentration of 1.0 g/L.
- Test performance:
- The preliminary test was performed at 50 ± 0.5°C for 5 days applying aqueous buffer solutions at pH 4, 7 and 9. Samples of the different aqueous extracts were collected at time 0, 2 hours, and after 5 days. Collected aliquots were further diluted and analysed by HPLC/UV allowing the detection of ≤ 10 % of the time 0 levels of the test substance in the buffer solutions.
- Transformation products:
- no
- Details on hydrolysis and appearance of transformation product(s):
- no significant hydrolysis observed, therefore no transformation products analyzed
- % Recovery:
- > 100
- St. dev.:
- 2.1
- pH:
- 4
- Temp.:
- 50 °C
- Duration:
- 5 d
- Remarks on result:
- hydrolytically stable based on preliminary test
- % Recovery:
- > 100
- St. dev.:
- 1.6
- pH:
- 7
- Temp.:
- 50 °C
- Duration:
- 5 d
- Remarks on result:
- hydrolytically stable based on preliminary test
- % Recovery:
- 98.4
- St. dev.:
- 98.4
- pH:
- 9
- Temp.:
- 50 °C
- Duration:
- 5 d
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 4
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 9
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Remarks on result:
- hydrolytically stable based on preliminary test
- Details on results:
- The average recoveries obtained in Tier 1 testing were always in the range of 98-102 %. Test analyte was hydrolyzed less than the 10 %. This indicated a sufficient hydrolytic stability of Guanylurea Phosphate in different buffer solutions after 5 days of incubation at 50 °C. No significant hydrolysis (≤ 10 %) was observed in the preliminary test at 50 °C for all tested pH values (4, 7, 9) of guanylurea phosphate. This indicates a half-life period of Guanylurea Phosphate at 25 °C of more than 1 year (t0.5 25°C > 1 year). Thus, the test substance Guanylurea Phosphate was considered to be hydrolytically stable and a further testing (Tier 2 experiments) (according to OECD guideline 111) was not required.
- Results with reference substance:
- No reference substance tested
- Validity criteria fulfilled:
- yes
- Conclusions:
- No significant hydrolysis (≤ 10 %) was observed in the preliminary test at 50 °C for all tested pH values (4, 7, 9) of guanylurea phosphate. This indicates a half-life period of Guanylurea Phosphate at 25 °C of more than 1 year (t0.5 25°C > 1 year). Thus, the test substance Guanylurea Phosphate was considered to be hydrolytically stable and a further testing (Tier 2 experiments) (according to OECD guideline 111) was not required.
- Executive summary:
The objective of the study was to assess the abiotic hydrolytic transformation of the test item in aquatic systems at three different pHs (4.0, 7.0, and 9.0). Experiments were performed to determine the rate of hydrolysis as a function of pH and temperature considering requirements of OECD Guideline 111. The preliminary test was performed at 50 ± 0.5°C for 5 days applying aqueous buffer solutions at pH 4, 7 and 9. Samples of the different aqueous extracts were collected at time-0, 2 hours, and after 5 days. Collected aliquots were further diluted and analysed by HPLC/UV allowing the detection of ≤ 10 % of the time-0 levels of the test substance in the buffer solutions.
The test lab successfully performed the hydrolysis testing for Guanylurea Phosphate in aquatic systems at three different pHs (4, 7, and 9) demonstrating the hydrolytic stability of the test substance after 5 days incubation at 50 °C (Tier 1). Thus, no further hydrolysis experiments (Tier 2) were deemed to be necessary. The half-life period for the analytes at 25 °C was assumed to be more than 1 year (t0.5 25°C > 1 year).
Reference
Description of key information
No significant hydrolysis (≤ 10 %) was observed in the preliminary test at 50 °C for all tested pH values (4, 7, 9) of guanylurea phosphate. This indicates a half-life period of Guanylurea Phosphate at 25 °C of more than 1 year (t 1/2 at 25°C). Thus, the test substance Guanylurea Phosphate was considered to be hydrolytically stable and a further testing (Tier 2 experiments) (according to OECD guideline 111) was not required.
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 1 yr
- at the temperature of:
- 25 °C
Additional information
Source: GLP-study
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.