Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 251-646-7 | CAS number: 33703-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Principles of method if other than guideline:
- The study was performed according to European standard test guideline, Directive 79/831/EWG, appendix V, part C, stand Nov 1989.
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 100 mg/L stock solution was prepared using the vehicle Cremophor RH 40 and mixing for 1020 minutes at 20 °C. The stock solution was stepwise diluted to form the desired test concentrations.
- Chemical name of vehicle: Cremophor RH 40
- Concentration of vehicle in test medium (stock solution): 100 mg/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: in-house culture originally obtained from Institut National de Recherche Chimique Appliquee, France
- Age at study initiation (mean and range, SD): 2-26h
- Feeding during test: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.69 mmol/L
- Test temperature:
- 20 °C
- pH:
- 7.7 - 8.1
- Dissolved oxygen:
- 7.6 - 9.3 mg/L
- Nominal and measured concentrations:
- Nominal: 0.049, 0.098, 0.195, 0.39, 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 20 Ml test vessels with 10 mL test solution
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: at the beginning and the end of the test (DO, pH). temperature continuously
OTHER TEST CONDITIONS
- Photoperiod: continuous dark
EFFECT PARAMETERS MEASURED: Immobilisation was recorded after 3, 6, 24 and 48h exposure time. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: At concentrations between 1.56 and 100 mg/l the test organisms swam partly at the surface.
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none - Validity criteria fulfilled:
- yes
- Conclusions:
- In a acute toxicity test under static conditions, Daphnia magna showed no mortality to DINA concentrations up to 50 mg/L. The 48h-EC50 value is determined to be > 100 mg/L.
Reference
Immobilised organisms after 48 hours (from 20 organisms / conc.):
nom. conc. (mg/l ) 0h 3h 6h 24h 48h
(control) - - - - -
(cont. solubilizer) - - - - -
100 - - - 3 3
50 - - - 1 1
25 - - - - 2
12.5 - - - - 2
6.25 - - - - 2
3.13 - - - - 2
1.56 - - - - 1
0.78 - - - - -
0.39 - - - - -
0.195 - - - - -
0.098 - - - - -
0.049 - - - - -
REMARK
Effect concentration is greater than the solubility of the substance in the test medium.
OXYGEN CONTROL (mg/l)
nom. conc. (mg/l ) 0h 48h
(control) 9.1 8.9
(cont. solubilizer) 9.0 7.6
100 8.5 8.2
50 8.8 8.2
25 9.0 8.2
12.5 9.0 8.2
6.25 9.1 8.9
3.13 9.2 8.9
1.56 9.2 8.9
0.78 9.2 8.9
0.39 9.2 8.9
0.195 9.3 9.0
0.098 9.3 9.0
0.049 9.3 9.0
pH CONTROL 0h 48h
(control) 8.0 7.9
(cont. solubilizer) 8.0 7.8
100 7.7 7.7
50 7.9 7.8
25 8.0 7.8
12.5 8.0 7.9
6.25 8.0 7.9
3.13 8.1 7.9
1.56 8.0 8.0
0.78 8.1 7.9
0.39 8.1 8.0
0.195 8.1 8.0
0.098 8.1 8.0
0.049 8.0 7.9
Description of key information
EC50 (48h) > 100 mg/L (nominal, vehicle used) for Daphnia magna (similar to OECD 202)
Key value for chemical safety assessment
Additional information
One study investigating the short-term toxicity of Diisononyl adipate (DINA) to aquatic invertebrates is available. The study was performed according to European standard test guideline, Directive 79/831/EWG, appendix V, part C, adopted in Nov 1989 (similar to OECD 202) under static conditions using Daphnia magna as test organism (BASF 1990). Concentrations between 0.049 and 100 mg/L were tested using a solubilizer (Cremophor RH 40). No mortality was observed in the control and treatments up to 50 mg/L during the exposure period of 48h. The 48h-EC50 is determined to be > 100 mg/L based on nominal concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)