Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 257-765-0 | CAS number: 52234-82-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
Skin irritation (OECD TG 404): not irritating
Eye irritation (similat to OECD TG 405): severely eye damaging
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well-documented, guideline-conform study report
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- GLP compliance:
- no
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Lot/batch No.of test material: WF 4746 - Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- Acclimatization period: at least 8 days before study start
Mean body weight: male 3.18 kg, females 2.92 kg
Singly housing in stainless steel wire mesh cages (floor area 40 x 51 cm).
The animals were identified via ear tattoo.
They were housed in fully air-conditioned rooms with a room temperature of 20 - 24°C and a relative humidity of 30 - 70%. The day/night rhythm was 12 h light and 12 h darkness.
The animals were offered a standardized animal laboratory diet (about 130 g/animal/day) as well as tap water (about 250 ml/animal/day). - Type of coverage:
- semiocclusive
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated skin of the animals
- Amount / concentration applied:
- 0.5 ml
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 30 - 60 min after removal of the test patches and 24 h, 48 h, 72 h after the beginning of the application
- Number of animals:
- 3
- Details on study design:
- Clipping of the fur at least 15 hours before study start.
Application area: 2.5 x 2.5 cm
Application site: upper third of the back or flanks
Removal of the test substance at the end of the application period with Lutrol and Lutrol/water (1:1).
Readings: 30 - 60 minutes after removal of the test patches and 24 h, 48 h and 72 h after beginning of application. - Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 24 - 72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 24 - 72 h
- Score:
- 0
- Max. score:
- 0
- Interpretation of results:
- GHS criteria not met
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- purity of the test substance not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- no
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Lot/batch No.of test material: C-001 388
- Trimethylolpropane tris (3-azirinyl) propanoate (CAS# 52234-82-9); (Paradox ID:A-11/08/91:01101) - Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: L.I.T. Laboratory Animals, Santa Clara, California
- Weight at study initiation: 2.5 - 3.5 kg - Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL
- Concentration (if solution): undiluted - Duration of treatment / exposure:
- 30 sec, 5 min, > 5 min (substance was not removed from the eye)
- Observation period (in vivo):
- 72 h
- Number of animals or in vitro replicates:
- 9 (in 3 rabbits the test material was washed off 30 sec after instillation, in 3 others the material was washed from the eye 5 min after Instillation and in the last 3, the compound was not washed from the eye.
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): in 3 rabbits the test material was washed off 30 sec after instillation, in 3 others the material was washed from the eye 5 min after Instillation and in the last 3, the compound was not washed from the eye
SCORING SYSTEM: The grading system was taken from the Draize Test. - Remarks on result:
- other: eyes were so severly damaged that scoring was not possible
- Irritant / corrosive response data:
- The eyes of all rabbits treated with the test substance, regardless of the washing conditions, were so severely damaged by the reading at 1 hour that scoring corneal and iris tissue was impossible. Maximum scores were recorded for conjunctival tissue 1, 4, 24, and 72 hours after compound treatment. Bleeding from the eyes of several rabbits was observed at 72 hours.
- Other effects:
- The observations were not continued beyond 72 hours, and all rabbits were sacrificed at that time.
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- The test substance is totally corrosive to the rabbit eye, even if it is washed out within 30 seconds of instillation.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irreversible damage)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin irritation/corrosion:
In a skin irritation study according to OECD Guideline 404, 0.5 mL of the test substance were applied to the back (fur clipped at least 15 h before application of the test substance) of one male and 2 female White Vienna rabbits to an area of 2.5 x 2.5 cm. As negative control untreated skin sites of the same animal were used. After 4 h with semi-occlusive coverage, the substance was removed with Lutrol and Lutrol/water (1:1). Skin reactions were assessed 30 - 60 minutes after removal of the patch and 24, 48 and 72 h after the beginning of application. After patch removal very slight erythema were recorded in 2 of 3 animals (intact skin), which were fully reversible within 24 hours. Therefore, the test substance was not irritant under these test conditions.
Eye irritation:
In an eye irritation study, 0.1 mL of the substance was instilled into the right eye of 9 New Zealand White rabbits. The left eye served as the untreated control. In three rabbits, the test substance was washed from the eye 30 seconds after instillation; in three others, the material was washed from the eye 5 minutes after instillation; and in the last three, the compound was not washed from the eye. Each eye was graded for ocular lesions 1, 4, 24, and 72 hours after instillation of the test substance. The grading system was taken from the Draize Test.
Moderate-to-severe lesions of the cornea, iris, and conjunctivae were seen in all rabbits treated with the test substance, regardless of wash treatment. Many corneal and iris reactions were difficult to score during the first few days due to extreme swelling of conjunctivae, resulting in closure of the eyelids. Generally, however, corneal opacity occurred within 24 hours. At 7 days after treatment, all 9 treated eyes had some degree of opacity over most or all of the corneal area. The density of these lesions ranged from translucent to opaque, with the worst cases appearing in the 5-minute wash group. Irises were also severely affected - two each in the 5-minute and no-wash groups showed no reaction to light. The remaining eyes in all groups had hemorrhaged irises. Conjunctival swelling, redness, and discharge were still extremely pronounced on Day 7.
The decision was made to sacrifice the rabbits on Day 7 since it appeared that healing, with a return to normal sight, was highly unlikely.
The test substance is considered to be a severe irritant to the rabbit eye.
Justification for classification or non-classification
Classification, Labelling, and Packaging Regulation (EC) No. 1272/2008
The available experimental test data are reliable and suitable for classification purposes under Regulation 1272/2008. As a result the substance is not considered to be classified for skin irritation, but has to be classified for causing severe eye damage (Cat. 1) under Regulation (EC) No. 1272/2008, as amended for the tenth time in Regulation (EU) No 2017/776.
The classification as severely eye damaging is consistant with the harmonised classification and labelling (CLP00).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)