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Diss Factsheets
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EC number: 471-480-0 | CAS number: 1645-83-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: 100 µL taken and added to appropriate vials to maintain conconcentation
- Sample storage conditions before analysis: stored at 2 - 10 ˚C and shipped to another facility at the end of the test (stability during storage and transport shown in another study) - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Vial filled with test solutions that had been purged for 30 or 60 minutes with clean air at a rate of approximately 100 mL/min. The test vessels were filled to the rim to minimize headspace and closed with transparent lids sealed with a silicone vacuum grease. This method was shown in another experiment to result in a saturated solution. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Source: Zodiac, LU Wageningen, the Netherlands
- Length at study initiation: 2.0 +/- 0.08 cm
- Weight at study initiation (mean and range, SD): 0.21 +/- 0.03
- Method of breeding: Fi from a single parent-pair bred in UV treated water
- Feeding during test: No
ACCLIMATION
- Acclimation period: At least 12 days
- Acclimation conditions: Same as test
- Type and amount of food: Pelleted fish food
- Feeding frequency: Daily
- Health during acclimation: Less than 5% for the 7 days prior to test initiation - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- Day 0: 20.6˚C
Day 4: 21.2 - 21.3˚C - pH:
- Day 0: 8.0
Day 4: 7.4 - 7.5 - Dissolved oxygen:
- Day 0: 6.8 - 8.8
Day 4: 5.2 - 6.8 - Nominal and measured concentrations:
- Nominal: 30 minute and 60 minute purge
Measured: 87 and 117 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 L
- Type: Closed (with transparent lids sealed with high vacuum grease)
- Material, size, headspace, fill volume: 9.8 liter test solution in 10 liter bottle
- Aeration: No
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: 0.15 g/L (7 fish per 9.8 L)
- Water parameters: Oxygen content, pH and temp measured at time 0 and end of test
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hour photoperiod daily
EFFECT PARAMETERS MEASURED: Mortality and other effects at 2.5, 24, 48, 72, and 96 hours; - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 117 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: None
- Other adverse effects control: No - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: 100%
- LC50: 0.15 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- In an acute aquatic toxicity study performed in accordance with OECD TG 203, the 96 hour LC50 for carp was determined to be > 117 mg/L based on mean measured concentrations.
Reference
Description of key information
The short-term toxicity of the substance to fish was determined in a study in accordance with OECD TG 203. In this study, the 96-h LC50 value is > 117 mg/L (mean measured concentration).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 117 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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