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EC number: 800-940-9 | CAS number: 35836-72-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 December 1996 – 17 January 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study meets OECD Guideline No 301F requirements.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Source of activated sludge: biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Aire)
Preconditioning: the sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 min, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.
Dry weight of suspended solids: 3.793 g/L
Concentration of sludge: 30 mg dw/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: the following stock solutions were prepared
Solution A: KH2PO4: 8.5 g/L, K2HPO4: 21.75 g/L, Na2HPO4.2H2O: 33.4 g/L, NH4Cl: 0.5 g/L
Solution B: CaCl2: 27.5 g/L
Solution C: MgSO4.7H2O: 22.5 g/L
Solution D: FeCl3.6H2O: 0.25 g/L, HCl conc.: one drop
Mineral medium was prepared by mixing 50 mL of solution A and 2000 mL of deionized water, adding 5 mL of each of the solutions B, C and D and making up to 5 L with deionized water.
- Source/preparation of dilution water: deionized water containing less than 10 mg/L dissolved organic carbon
- Test temperature: 22 °C
TEST SYSTEM
- Culturing apparatus: flasks of the volumetric respirometer (SAPROMAT)
- Number of culture flasks/concentration: two
- Details of trap for CO2 if used: evolved CO2 was absorbed by sodalime pellets
MEASURING EQUIPMENTS:
- Respirometer: SAPROMAT D 12 (J. M. VOITH GmbH, Heidenheim, Germany)
SAMPLING
- Sampling frequency: everyday the oxygen consumption of each flask was recorded and correct temperature and stirring were checked. At the end of the test period (normally 28 days), the pH of each flask was measured again.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes; containing inoculum only
- Procedure control: yes; containing reference substance (aniline) with inoculated medium
- Toxicity control: yes; containing test material and reference substance (sodium benzoate) with inoculated medium - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 100 mg/L; purity: min. 99.0 %; source: Fluka, Switzerland
- Preliminary study:
- no data
- Test performance:
- no data
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 78
- Sampling time:
- 28 d
- Details on results:
- Initial test material concentration: 100 mg/L
Theoretical oxygen demand (ThOD) = 2.61 mg O2/mg
% biodegradation on Day 7: > 10 %
% biodegradation on Days 17 > 60 %
% biodegradation on Days 28 = 78 % - Results with reference substance:
- Theoretical oxygen demand (ThOD) = 1.67 mg O2/mg
% biodegradation on Day 5: > 60 %
% biodegradation on Days 14 = 83 % - Validity criteria fulfilled:
- yes
- Remarks:
- % degradation in reference material reached the pass levels by Day 14; oxygen uptake of inoculum blank was < 60 mg O2/L; pH of the test solutions at the end of the test was 7.40-7.44; toxicity control showed no toxicity to microorganisms
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Nopyl acetate was readily biodegradable in a test carried out according to OECD Guideline No 301 F.
- Executive summary:
In a ready biodegradation study performed according to OECD Guideline No 301 F and GLP, nopyl acetate was tested at a concentration of 100 mg/L and the inoculum was activated sewage sludge, domestic. The degradation of the test material was assessed by the determination of the oxygen consumption. The test treatments, inoculum blank, procedure control and toxicity control were measured in duplicates.
At 100 mg/L test concentration, greater than 10 % degradation was reached on Day 7 and greater than 60 % biodegradation was reached on Day 17. The pass level for ready biodegradability was reached in the required 10-d window within the 28-d period of the test: 78% degradation on Day 28.
The reference material (sodium benzoate) reached greater than 60 % biodegradation within 5 days. The biodegradation curves obtained with the reference substance alone and with test material+reference substance show no toxic effect on the micro-organisms. Hence, it met the validity criteria for reference material and toxicity control.
Therefore, nopyl acetate was readily biodegradable.
Reference
Table 5.2.1/2: Test material: Biological Oxygen Demand (BOD, mg O2/L, adjusted to nominal concentrations)
Days |
5 |
7 |
14 |
17 |
21 |
28 |
||
BOD Sludge |
1st flask |
B1 |
22.0 |
23.0 |
31.0 |
32.0 |
37.0 |
42.0 |
2nd flask |
B2 |
22.0 |
23.0 |
30.0 |
31.0 |
37.0 |
44.0 |
|
mean |
B |
22.0 |
23.0 |
30.5 |
31.5 |
37.0 |
43.0 |
|
BOD Test substance |
1st flask |
C1 |
39.3 |
42.1 |
176.2 |
207.6 |
240.9 |
245.6 |
2nd flask |
C2 |
39.4 |
77.6 |
177.1 |
217.3 |
242.2 |
248.9 |
|
1st flask corrected |
C1-B |
17.3 |
19.1 |
145.7 |
176.1 |
203.9 |
202.6 |
|
2nd flask corrected |
C2-B |
17.4 |
54.6 |
146.6 |
185.8 |
205.2 |
205.9 |
|
% Degradation |
1st flask |
D1 |
7 |
7 |
56 |
67 |
78 |
78 |
2nd flask |
D2 |
7 |
21 |
56 |
71 |
79 |
79 |
|
mean |
D |
7 |
14 |
56 |
69 |
78 |
78 |
B = (B1 + B2) /2;
D1 = 100*(C1 - B)/ThOD*[S]; D2 = 100*(C2 - B)/ThOD*[S]; D = (D1 + D2)/2
[S]: Initial test substance concentration (mg/L)
Table 5.2.1/3: Reference substance (sodium benzoate): Biological Oxygen Demand (BOD, mg O2/L, adjusted to nominal concentrations)
Days |
5 |
7 |
10 |
14 |
21 |
28 |
||
BOD Sludge |
1st flask |
B1 |
22 |
23 |
26 |
31 |
37 |
42 |
2nd flask |
B2 |
22 |
23 |
24 |
30 |
37 |
44 |
|
mean |
B |
22 |
23 |
25 |
30.5 |
37 |
43 |
|
BOD reference substance |
1st flask |
A1 |
143 |
152.9 |
164.9 |
172.9 |
184.9 |
195.9 |
2nd flask |
A2 |
136 |
145 |
154.9 |
163.9 |
171.9 |
178.9 |
|
1st flask corrected |
A1-B |
121 |
129.9 |
139.9 |
142.4 |
147.9 |
152.9 |
|
2nd flask corrected |
A2-B |
114 |
122 |
129.9 |
133.4 |
134.9 |
135.9 |
|
% Degradation |
1st flask |
D1 |
73 |
78 |
84 |
86 |
89 |
92 |
2nd flask |
D2 |
68 |
73 |
78 |
80 |
81 |
82 |
|
mean |
D |
71 |
76 |
81 |
83 |
85 |
87 |
B = (B1 + B2)/2;
D1 = 100*(A1 - B)/ThOD*[S]; D2 = 100*(A2 - B)/ThOD*[S]; D = (D1 + D2)/2
[S]: Initial test substance concentration (mg/L)
Description of key information
Screening for ready biodegradability was performed by a manometric respiratory test.
Nopyl acetate is readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Key study conducted according to OECD 301 F Guideline and GLP without deviation.
Nopyl acetate was tested at a concentration of 100 mg/L. The inoculum derived from domestic sewage sludge. Degradation was assessed by measurement of oxygen consumption.
The pass level for ready biodegradability was reached in the required 10-d window within the 28-d period of the test: 78% degradation on Day 28. This result can be used as key value for chemical safety assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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