Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 950-299-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 October until 25 November 2009
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Modified guideline study, to GLP
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- Minor deviation: Ammonium chloride was omitted from the medium to prevent oxygen consumption due to nitrification (the omission does not result in nitrogen limitation as shown by the biodegradation of the reference compound).
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO DIS 9408 (Ultimate Aerobic Biodegradability - Method by Determining the Oxygen Demand in a Closed Respirometer)
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):Not applicable
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: secondary activated sludge, predominantly domestic wastewater, preconditioned to reduce the endogenous respiration rates
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): wastewater treatment plant Nieuwgraaf, Duiven, The Netherlands- Pretreatment: 400 mg dry weight/l of activated sludge was aerated for 1 week- Concentration of sludge: 400 mg/l- Initial cell/biomass concentration: no data- Water filtered: no
- Duration of test (contact time):
- >= 28 d
- Initial conc.:
- ca. 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS- Composition of medium: Water contained 8.5 mg/L KH2PO4, 21.75 mg/L K2HPO4, 33.3 mg/L Na2HPO4.2H2O, 22.5 mg/L MgSO4.7H2O, 27.5 mg/L CaCl2, 0.25 mg/L FeCl3.6H2O; sodium acetate stock solution concentration: 1.0 g/L; test substance stock suspension concentration 1.0 g/L in deionized water, prepared with polyalkoxylate alkylphenol (Agnique) and using ultrasonification (10 minutes at 200 W using a Vibra cell ultrasonic processor).- Additional substrate: Agnique- Test temperature: 22-24oC- pH: 7.0-7.1- pH adjusted: no- Aeration of dilution water: no- Suspended solids concentration: no data- Continuous darkness: yesTEST SYSTEM- Culturing apparatus: 0.3 L BOD (biological oxygen demand) test bottles - Number of culture flasks/concentration: 10- Measuring equipment: oxygen electrode (WTW Trioxmatic EO 200) and meter (WTW OXI 530)- Other: BOD test bottles were completely filled and free of air bubbles; glass stoppers were usedSAMPLING- Sampling frequency: weekly- Sampling method: from duplicate bottlesCONTROL AND BLANK SYSTEM- Inoculum blank: no- Abiotic sterile control: no- Toxicity control: not relevant- Other: 10 bottles contained only inoculum; 10 bottles contained inoculum and Agnique; 10 bottles contained inoculum and test substance suspended with Agnique; 6 bottles contained inoculum and sodium acetateSTATISTICAL METHODS: None
- Reference substance:
- acetic acid, sodium salt
- Preliminary study:
- No data
- Test performance:
- None reported
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 64
- Sampling time:
- 28 d
- Remarks on result:
- other: Readily biodegradable; polychlorinated tetradecane (45% chlorinated)
- Details on results:
- Biodegradation for the C14 chlorinated paraffin was:
0 days - 0%
7 days - 3%
14 days - 14%
21 days - 32%
28 days - 64%
42 days - 67% - Results with reference substance:
- The biodegradation of the reference compound, sodium acetate, was >60% at day 7 and 71% at day 14.
- Validity criteria fulfilled:
- yes
- Remarks:
- Test validity demonstrated by oxygen concentrations of >0.5 mg/L in all bottles during the test period and by an endogenous respiration of 1.1 mg/L at day 28.
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Conclusions:
- In a modified guideline study, to GLP, the extent of degradation of a polychlorinated tetradecane (45% chlorinated) by aerated secondary activated sludge, as measured by dissolved oxygen concentration in a Closed Bottle test, was 64% after 28 days.
It was considered that the number of bottles necessary to evaluate the 10-d window would cause the test to become too unwieldy therefore a 14-day window was used for this closed bottle test as described in the OECD 301 guideline. The degree of biodegradation reached 10% on Day 14. By Day 28 it has surpassed the 60% pass level within the 14-day window. - Executive summary:
In a GLP study, the biodegradation of a polychlorinated tetradecane (45% chlorinated) was determined by measuring oxygen consumption in a Closed Bottle test, where a concentration of 2 mg/L was incubated in the dark for at least 28 days with secondary activated sludge derived from a plant treating predominantly domestic wastewater. Test procedure deviations from OECD Guideline 301 D and EU Method C.4 -E were minor. Two duplicate bottles from each group were withdrawn for analyses of the dissolved oxygen concentration at day 7, 14, 21, 28 and 42.
The extent of biodegradation at day 0, 7, 14, 21, 28 and 42 was 0, 3, 14, 32, 64, and 67%, respectively. Therefore, this C14 chlorinated paraffin (45% chlorination) can be considered as readily biodegradable over a 28-day period (and not persistent).
Reference
Description of key information
Readily biodegradable but failing 10 day window. Results indicate the substance is not persistant.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable but failing 10-day window
- Type of water:
- freshwater
Additional information
It was considered that the number of bottles necessary to evaluate the 10-d window would cause the test to become too unwieldy therefore a 14-day window was used for this closed bottle test as described in the OECD 301 guideline. The degree of biodegradation surpassed 10% on Day 14, by Day 28 it has surpassed the 60% pass level and thus regarded as readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.