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EC number: 213-208-3 | CAS number: 930-02-9
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-01-19 to 1989-03-20
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study. Only four bacterial strains tested (S. typh. TA98, TA100, TA 1535, TA1537). No GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1988
- Deviations:
- no
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1-(vinyloxy)octadecane
- EC Number:
- 213-208-3
- EC Name:
- 1-(vinyloxy)octadecane
- Cas Number:
- 930-02-9
- Molecular formula:
- C20H40O
- IUPAC Name:
- 1-(ethenyloxy)octadecane
- Test material form:
- other: colourless liquid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S-9 mix
- Test concentrations with justification for top dose:
- 0; 20; 100, 500; 2500; 5000 µg/plate
- Vehicle / solvent:
- DMSO
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene (2-AA)
- Remarks:
- with S-9 mix; TA98, TA100,TA1535, TA1537
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)
- Remarks:
- without S-9 mix; TA100, TA1535
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylenediamine
- Remarks:
- without S-9 mix; TA98
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without S-9 mix; TA1537
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: Standard plate test
DURATION
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3 plates per dose and control
METHOD OF APPLICATION: Preincubation test
DURATION
- Preincubation period: 20 min at 37°C
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3 plates per dose and control
DETERMINATION OF CYTOTOXICITY
- Method: background growth - Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results . - Statistics:
- NA
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- The slight but not dose-dependent increase in the number of his+ revertants using TA 1537 in the 1st experiment could not be confirmed either in a additional standard plate test or in a preincubation assay; therefore the findings of this 1st experiment must be regarded as incidental.
Toxicity: No bacteriotoxic effect (reduced his- background growth) was observed.
Solubility: Complete solubility of test substance in DMSO. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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