Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-410-5 | CAS number: 140-29-4
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Klimish 2f Study was performed using an accepted calculation method
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Composition of the essential oil of Lepidium meyenii (Walp.)
- Author:
- Tellez M.R., Khan I.A., Kobaisy M., Schrader K., Dayan F.E. and Osbrink W.
- Year:
- 2 002
- Bibliographic source:
- Phytochemistry, 61(2), 149 - 155
- Reference Type:
- other: Databank
- Title:
- No information
- Author:
- Beilstein
- Year:
- 2 010
- Bibliographic source:
- Beilstein database, CAS n° 140-29-4
Materials and methods
- Principles of method if other than guideline:
- Rapid bioassay utilizing 96-well cell culture plates for screening herbicides and other phytotoxins for selective toxicity toward cyanobacteria and algae (according to Schrader et al. (1997))
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Phenylacetonitrile
- EC Number:
- 205-410-5
- EC Name:
- Phenylacetonitrile
- Cas Number:
- 140-29-4
- Molecular formula:
- C8H7N
- IUPAC Name:
- 2-phenylacetonitrile
- Details on test material:
- - Name of test material (as cited in study report):phenylacetonitrile
No more data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- No data
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): pentane
No more data
Test organisms
- Test organisms (species):
- other: Oscillatoria perornata and Selenastrum capricornutum
- Details on test organisms:
- No data
Study design
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 4 d
- Post exposure observation period:
- No data
Test conditions
- Hardness:
- No data
- Test temperature:
- No data
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- 0.0117, 0.117, 1.17, 11.7 and 117 µg/mL
- Details on test conditions:
- No data
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 1 d
- Dose descriptor:
- other: Lowest Complete Inhibition Concentration (LCIC)
- Effect conc.:
- 117 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: Absorbance at 650 nm
- Remarks on result:
- other: Oscillatoria perornata
- Duration:
- 4 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 117 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: Absorbance at 650 nm
- Remarks on result:
- other: Selenastrum capricornutum
- Details on results:
- No data
- Reported statistics and error estimates:
- No data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- Under the test conditions a lowest complete inhibition concentration (LCIC) of 117 µg/mL was found after 1 day for the cyanobacterium Oscillatoria perornata, whilst a NOEC of 117 µg/mL was observed after 4 days for the green alga Selenastrum capricornutum. Thus, phenylacetonitrile may be a selective growth inhibitor for Oscillatoria perornata, but based on these results the substance cannot be classified according to the CLP regulation (EC) N° 1272/2008.
- Executive summary:
The authors tested the toxicity of phenylacetonitrile (CAS n° 140-29-4) to the cyanobacterium Oscillatoria perornata and the green alga Selenastrum capricornutum in a 4d growth inhibition test. This test was a rapid bioassay test according to Schrader et al. (1997) which utilizes 96-well cell culture plates for screening substances for selective toxicity toward cyanobacteria and algae by measuring the absorbance at 650 nm. The concentrations tested were 0.0117, 0.117, 1.17, 11.7 and 117 µg/mL. Total exposure duration was 4d and every 24h the absorbance was measured of the different samples.
Under these test conditions, a lowest complete inhibition concentration (LCIC) of 117 µg/mL was found after 1 day for Oscillatoria perornata, whilst a NOEC of 117 µg/mL was observed after 4 days for Selenastrum capricornutum. In conclusion, phenylacetonitrile is selectively toxic towards Oscillatoria perornata compared to Selenastrum capricornutum. Thus, phenylacetonitrile may be a selective growth inhibitor for Oscillatoria perornata, but based on these results the substance cannot be classified according to the CLP regulation (EC) N° 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
