2,5-xylidine

Endpoint summary

Description of key information

Short-term toxicity to fish:

Experiment was performed to determine the short-term toxicity of the test material, according to OECD Guideline 203 (Fish, Acute Toxicity Test) in a static system for the duration of 96h. After the exposure of the test chemical to the fish for 96 hours, the LC50 was determined at >110 mg/L. Since the value is greater than 110mg/L, the test chemical is considered to be not classified.

Short-term toxicity to aquatic invertebrates:

Study was conducted to determine the effect of test chemical on the immobility rate of daphnia. A 48 hours study was conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) to determine the toxicity of the test material on daphnia in a static system. After 48 hours of exposure, EC50 was determined at 18 mg/L. Hence on the basis of above data, test chemical was classified under aquatic chronic 3.

Toxicity to aquatic algae:

Study was conducted to assess the effect of test chemical on the growth rate of green alga. Test was conducted according to OECD Guideline 201 (Alga, Growth Inhibition Test) for determining toxicity of the test chemical on aquatic alga in a static medium. After 72 h of study, effective concentration was determined to be at 30 mg/L on the basis of growth rate inhibition, while NOEC was determined at 2.0 mg/L. Based on the EC50 value, the test chemical is consider to be toxic and can be consider to be classified under aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to micro-organisms:

Short-term toxicity of the test chemical on micro-organisms (Photobacterium phosphoreum)for 30 min, was determined to be 22 mg/L.

Additional information

Short-term toxicity to fish:

Based on the experimental data, toxicity of test chemical was determined on the basis of mortality of fishes. The studies are summarized as below:

First experiment was performed to determine the short-term toxicity of the test material, according to OECD Guideline 203 (Fish, Acute Toxicity Test) in a static system for the duration of 96h. After the exposure of the test chemical to the fish for 96 hours, the LC50 was determined at >110 mg/L. Since the value is greater than 110mg/L, the test chemical is considered to b not classified.

Second experiment was performed from secondary source to support the first experimental study for determining the toxicity of test chemical.

Aim of second experimental study was to determine the effect of test chemical to Oryzias latipes for 48h. After 48h of exposure, the LC50 value was determined to be 239 mg/L. Based on the above value, the chemical is considered to be not classified.

Further, the third experimental study was conducted to determine the toxicity of the test chemical on the mortality of fishes.

Aim of the third study was to determine the effect of test chemical on the mortality of fishes. The LC50 value was determined to be 58 mg/l. Based on the above value and according to the classification, the chemical is classified under aquatic chronic 3.

Hence, based on the maximum experimental values, it can be concluded that the chemical is not classified.

Short-term toxicity to aquatic invertebrates:

Based on the experimental data, toxicity of test chemical was determined on the basis of immobility rate of aquatic invertebrates. The studies are summarized as below:

First study was conducted to determine the effect of test chemical on the immobility rate of daphnia. A 48 hours study was conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) to determine the toxicity of the test material on daphnia in a static system. After 48 hours of exposure, EC50 was determined at 18 mg/L. Hence on the basis of above data, test chemical was classified under aquatic chronic 3.

Second study was performed from secondary source to support the first experimental study for determining the toxicity of test chemical.

Test was performed to determine the toxicity of the test chemical on aquatic invertebrates (crustaceans). The value of LC50 was determined to be 62 mg/L, based on which the test chemical was considered to be toxic and can be classified under aquatic chronic 3.

Thus, based on the above experimental data, it can be concluded that the chemical is toxic and can be considered to be classified under aquatic chronic 3.

Toxicity to aquatic algae:

Based on the experimental data, toxicity of test chemical was determined on the basis of growth rate of aquatic algae. The studies are summarized as below:

First study was conducted to assess the effect of test chemical on the growth rate of green alga. Test was conducted according to OECD Guideline 201 (Alga, Growth Inhibition Test) for determining toxicity of the test chemical on aquatic alga in a static medium. After 72 h of study, effective concentration was determined to be at 30 mg/L on the basis of growth rate inhibition, while NOEC was determined at 2.0 mg/L. Based on the EC50 value, the test chemical is consider to be toxic and can be consider to be classified under aquatic chronic 3 category as per the CLP classification criteria.

First experimental study for the test chemical was further supported by second experimental study from secondary source.

Aim of second study was to determine the effect of test chemical on aquatic algae. After the exposure, LC50 value was determined to be 44mg/L. Based on the above value, the chemical is considered to be toxic and can be classified under aquatic chronic 3 category.

Again third study was conducted for testing the toxicity of test chemical on aquatic algae.

Aim of third experiment was to determine the toxicity of the test chemical on the algal species (Tetrahymena pyriformis). After 48 hours of exposure, effective concentration (EC50) was determined to be 268 mg/L. Hence, based on the above value chemical is considered to be not classified.

Thus, based on the maximum experimental values, it can be concluded that the chemical is toxic can be classified under aquatic chronic 3 category.

Toxicity tomicro-organisms:

Experiment was conducted to determine short-term toxicity of the test chemical on micro-organisms (Photobacterium phosphoreum)for 30 min. After the exposure, EC50 was determined at 22 mg/L.