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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

Data have been obtained from secondary source (EPA databank), including several pubblication.

For long-term toxicity to invertebrates only data for fresh water are available.

NOEC for freshwater crustaceans of 2000 µg/L has been choosen as the most relevant and conservative data.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
2 000 µg/L

Additional information


The most relevant and conservative study defines a mortality endpoint NOEC of 2000 µg/l, based on reproduction effects. This value was not measured by chemical analysis. 2,4 -dinitrophenol with a purity of was tested on Daphnia magna (Water Flea) in a renewal condition. The exposure duration was 21 days. Two replicates were done. Temperature, pH and dissolved oxigen were measured during the test. Furthermore, mortality was observed.