4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with biphenyl-4-ol

Long-term toxicity to fish

Description of key information

PNEC water can be derived based on the available short-term toxicity tests, and whether this test is necessary to be performed will depend on the testing result of long-term toxicity test of Daphnia which has been proposed or the CSA result.
In addition, a feasibility study was carried out for the OECD 234 study (see supporting study).
The use of solvents in tests assessing reproductive and endocrine potentials can cause long term effects of the solvents on these endpoints. In general, solvents can be used to provide a vehicle in which poorly soluble substances can be dissolved. However, the OECD guideline 234 gives clear advice to avoid the use of a solvent:
“33. Where a solvent is used, its final concentration should not be greater than 0.1 ml/L (36) and it should be the same concentration in all test chambers, except the dilution water control. However, every effort should be made to avoid the use of such solvent or keep solvent’s concentrations to a minimum.”
“16. When a solvent is used it should have no statistical significant effect on survival and should not produce any endocrine disrupting effects or other adverse effects on the early-life stages as revealed by a solvent control.”
The use of solvents can have adverse outcomes on the endocrine endpoints of the sexual development of fish during the fish sexual development test.
The literature review strongly suggests avoiding the use of solvents, such as DMF or DMSO, in the fish sexual development test, due to possible effects of the solvents in the endocrine endpoints which are assessed in this test.
Therefore it can be concluded that it is not feasible to conduct a Fish Sexual Development Test with the test substance Resin E-200.