1,1-dimethoxytrimethylamine

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

Relative toxicities of chemicals to the earthworm Eisenia foetida

Author:

Roberts, B.L. and Dorough, H.W.

Year:

1984

Bibliographic source:

Environmental Toxicology and Chemistry 3: 67-78

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Principles of method if other than guideline:

Acute filter paper test with Eisenia fetida

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

IUPAC Name:

methanol

Details on test material:

- Name of test material (as cited in study report): Methanol
- Analytical purity: analytical grade

Sampling and analysis

Analytical monitoring:

not specified

Test substrate

Vehicle:

yes

Details on preparation and application of test substrate:

- Method of application to filter paper (if used): Methanol was dissolved in acetone, or other suitable solvent, and 1 mL of the solution was pipetted into each vial. A stream of warm air was directed into the vial to aid in evaporation of the solvent. After the vials had been under the stream of warm air for 3 min, they were air-dried for an additional 2 h to remove all remaining solvent. Then, 1 mL of water was added to each vial to moisten the paper and one earthworm was placed into each vial.
- Controls: Control vials received only the 1 mL of acetone
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone, or other suitable solvent
- Evaporation of vehicle before use: yes; a stream of warm air was directed into the vial to aid in evaporation of the solvent

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: Bert's Bait Farm, Irvine, KY, USA
- Age at test initiation (mean and range, SD): Mature worms showing a developed clitellum
- Weight at test initiation (mean and range, SD): 370 to
450 mg


ACCLIMATION
- Acclimation conditions (same as test or not): worms were housed in Nalgene boxes filled with moist peat moss and rabbit manure, and stored at 18°C. Calcium carbonate was added as necessary to maintain soil pH above 5.5

Study design

Study type:

laboratory study

Substrate type:

filter paper

Limit test:

no

Total exposure duration:

48 h

Test conditions

Details on test conditions:

TEST SYSTEM
- Test container (material, size): Glass shell vials (22 mm x 85 mm) (Research Products International, Mt. Prospect, IL, USA) were lined with Whatman No. 1 filter paper strips (9.5 cm x 6.8 cm, surface area, 65 cm 2) and placed in cardboard scintillation vial trays. Vials were capped.
- No. of organisms per container (treatment): 1
- No. of replicates per treatment group: 10
- No. of replicates per control: 1
- a minimum of 100 earthworms was tested in order to determine the LC50

OTHER TEST CONDITIONS
- Photoperiod: dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality

VEHICLE CONTROL PERFORMED: yes


TEST CONCENTRATIONS
- Spacing factor for test concentrations: geometric series of five to seven concentrations
- Range finding study
- Test concentrations: 0.1, 1, 10, 100, 1000 µg/cm2 (nominal)
- Results used to determine the conditions for the definitive study: The approximate lethal concentration was considered to be the lowest exposure that killed 50 % or more of the earthworms.

Nominal and measured concentrations:

0.1, 1, 10, 100, 1000 µg/cm2 (nominal)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 mg/cm²

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality