Benzyldimethyl[2-[(1-oxoallyl)oxy]ethyl]ammonium chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25/03/1993-29/04/1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 7827 (Evaluation in an Aqueous Medium of the "Ultimate" Aerobic Biodegradability of Organic Compounds - Method by Analysis of Dissolved Organic Carbon (DOC))

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzyldimethyl[2-[(1-oxoallyl)oxy]ethyl]ammonium chloride

EC Number:

256-283-8

EC Name:

Benzyldimethyl[2-[(1-oxoallyl)oxy]ethyl]ammonium chloride

Cas Number:

46830-22-2

Molecular formula:

C14H20NO2.Cl

IUPAC Name:

benzyldimethyl[2-(prop-2-enoyloxy)ethyl]azanium chloride

Details on test material:

- Name of test material (as cited in study report): ADAMQUAT BZ 80
- Physical state: : liquid
- Analytical purity: Purity: 78.5% (by weight in water)
- Lot/batch No.: OP 611/613/615/616

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Secondary effluent from a biologic, municipal wastewater treatment plant.
- Sampling site: Versailles
- Concentration : 23.8 mg/l related to DOC (Dissolved Organic Carbon)
- Preparation of inoculum for exposure: The effluent was collected on the start-up day and was centrifuged at 20°C during 20 min at 4000G to concentrate it by about a factor of 5.
- Initial cell/biomass concentration: : 2X10E6 bact/ml.

Duration of test (contact time):

ca. 35 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

ca. 7.2 g/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

ca. 3.57 g/L

Based on:

COD

Details on study design:

TEST CONDITIONS
- Composition of medium: For 1 litre:
1-10 ml of solution a: 8.5g KH2PO4, 21.75g K2HPO4, 33.4 g Na2HPO4,2H2O, 0.5g NH4Cl, q.s.p 1000ml ultrapure water.
2-1 ml of solution b: 22.5g MgSO4,7H2O in 1000 ml of ultrapure water.
3-1 ml of solution c: 27.5g CaCl2 in 1000 ml of ultrapure water.
4-1 ml of solution d: 0.25g FeCl3,6H2O in 1000 ml of ultrapure water , this solution was prepared at the beginning of the test.
5-800 ml of pure water.
6- ultrapure water q.s.p 1000 ml.
- Additional substrate: No
- Test temperature: 20-28±1°C
- pH: not specified
- pH adjusted: not specified
- Suspended solids concentration: not specified
- Continuous darkness: not specified

TEST SYSTEM
- Culturing apparatus: 500 ml glass Erlenmeyer flasks stoppered with cotton wool wrapped in sterilized gauze.
- Number of culture flasks/concentration: 3
- Test performed in closed vessels due to significant volatility of test substance: no

ANALYTICAL PARAMETER: Measure of dissolved organic carbon.

SAMPLING
- Sampling frequency: 7, 14, 21, 28, 35 days.
- Sampling method: DOC analyser

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Test performance:

No inhibition of the inoculum was observed.

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

ca. 23

Sampling time:

7 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

ca. 21

Sampling time:

14 d

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

ca. 26

Sampling time:

21 d

% Degradation 4

Parameter:

% degradation (DOC removal)

Value:

ca. 23

Sampling time:

28 d

% Degradation 5

Parameter:

% degradation (DOC removal)

Value:

ca. 30

Sampling time:

35 d

Details on results:

The method was applied with respect to its quality criteria:
The difference of extremes of replicate values of the removal of test chemical was less than 20%.

BOD5 / COD results

Results with reference substance:

Control substance: Benzoic acid, sodium salt
Kinetic : 7 day(s) = 100 %
28 day(s) = 100 %

Any other information on results incl. tables

The percentage degradation of the reference substance has reached a level of 100% by 14 days, which is above the level of 60% for ready biodegradability.

Biodegradation Dt (%)

Series	day	0	7	14	21	28	35
Control	Fc1 Fc2 Mean Fc	0 0 0	100 100 100	100 100 100	100 100 100	100 100 100	100 100 100
Substance	Ft1 Ft2 Ft3 Mean Ft	0 0 0 0	29 22 17 23	29 24 9 21	31 30 17 26	25 26 17 23	38 26 26 30
Sterile	Fs	0	-4	-7	-9	-13	-13
Inhibition	Fi	0	71	76	75	76	76

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

Under the test conditions, the percentage of biodegradation of the ADAMQUAT BZ 80 was 23% in 28 days, therefore, the substance is not considered to be inherently biodegradable.