3-diethylaminophenol

Endpoint summary

Additional information

Short-term toxicity to fish :

Based on the various studies available with Klimish rating 2 for the target substance CAS NO 91-68-9 and read across substances and based on the category approach of organic functional group along with similar mechanistic approach and having structural similarities defined by QSAR toolbox. The results is summarized as follows

 

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Remarks
1	LC50	34.03607mg/l	mortality	48 hrs	Oryzias latipes	Predicted data for target chemical
2	LC50	32.13076mg/l	mortality	48hrs	Pimephales promelas	Predicted data for target chemical
3	LC50	24.43437 mg/l	mortality	96 hrs	Oncorhynchus mykiss	Predicted data for target chemical
4	LC50	17.07mg/l	mortality	96 hrs	Pimephales promelas	Predicted data for target chemical
5	LC50	56.5 mg/l 72.6 mg/l 88.6 mg/l	mortality	96 hrs 48 hrs 24 hrs	Pimephales promelas	Data from publication for CAS NO 108-46-3
6	LC50	60 mg/l	mortality	96 hrs	Pimephales promelas	Data from publication for CAS NO 108-46-3

 

By applying weight of evidence approach to the studies summarized in the above table it can be observed that the LC50 values was found to be in range of 17.07 to 88.6 mg/l in 96, 48 and 24 hrs on the basis of mortality effect .

But the BCF value of 3-diethylaminophenol reported was not >= 2000 as well as the substance was found to be rapidly degradable

Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that 3-diethylaminophenol does not exhibit short term toxicity to fish (Oryzias latipes,Pimephales promelas and Oncorhynchus mykiss)

Long-term toxicity to fish :

According to annex IX of column I,Long-term toxicity testing on fish need not to be conducted as it is already provided as part of Annex VIII requirements.Therefore this study was considered for waiver.

Short-term toxicity to aquatic invertebrates :

Based on the various studies available with Klimish rating 2 for target chemical CAS NO 91-68-9 and the read across substances and based on the category approach of organic functional group along with similar mechanistic approach and having structural similarities defined by QSAR toolbox. The results is summarized as follows

 

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Remarks
1	EC50	30.87743 mg/l	mortality	24 hrs	Daphnia magna	Predicted data for target chemical
2	EC50	12.68779mg/L.	mortality	48 hrs	Daphnia magna	Predicted data for target chemical
3	LC50	42.2 mg/L.	mortality	96 hrs	Palaemonetes pugio	Data from publication for CAS no 108-46-3
4	LT50	>56 mg/L.	mortality	96 hrs	Crangon septemspinosa	Data from publication for CAS 108-46-3

 

By applying weight of evidence approach to the studies summarized in the above table it can be observed that EC50 value is expected to be in range of 12 to 42 mg/l and LT50 value was found to be >56 mg/l in a 24 to 96hrs study on the basis of mortality effect. But the BCF value of 3-diethylaminophenol reported was not >= 2000 as well as the substance was found to be rapidly degradable.Thus considering the CLP Criteria for aquatic classification of the substance it is concluded that the substance 3-diethylaminophenol is not toxic to aquatic invertebrate (Daphnia magna, Palaemonetes pugio and Crangon septemspinosa)

Long-term toxicity to aquatic invertebrates :

According to column 1 of Annex IX;Long-term toxicity testing on invertebrate was considered for waiver since the information on acute toxicity has already been provided as a part of Annex VII requirements.

Toxicity to aquatic algae and cyanobacteria :

Based on the various studies available with Klimish rating 2 for the target substance CAS NO 91-68-9 based on the category approach of organic functional group along with similar mechanistic approach and having structural similarities defined by QSAR toolbox. The results is summarized as follows

 

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Remarks
1	EC50	15.0057 mg/l	growth rate	72 hrs	Pseudokirchnerella subcapitata	Predicted data for target chemical
2	EC50	10.75694 mg/l	Cell changes	72 hrs	Pseudokirchnerella subcapitata	Predicted data for target chemical
3	EC50	19.86983mg/l	growth inhibition	N/A	Pseudokirchnerella subcapitata	Data from DEPA
4	EC50	25.321mg/l	no data	96 hrs	green algae	Data from publication form ECOSAR

 

By applying weight of evidence approach to the studies summarized in the above table it can be observed that EC50 value is expected to be in range of10.75694 to25.321 mg/l in 72 hrs to 96 hrs study.

But the BCF value of 3-diethylaminophenol reported was not >= 2000 as well as the substance was found to be rapidly degradable.

Thus considering the CLP Criteria for aquatic classification of the substance , it is concluded that 3-diethylaminophenol does not exhibit toxicity to aquatic algae (green algae, Pseudokirchnerella subcapitata)

Toxicity to microorganisms :

Based on the various studies available with Klimish rating 2 for the target substance CAS NO 91-68-9 and read across substances and based on the category approach of organic functional group along with similar mechanistic approach and having structural similarities defined by QSAR toolbox. The results is summarized as follows

 

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Remarks
1	EC50	187.1003mg/l	growth	12 hrs	Escherichia coli	Predicted data for target chemical
2	IGC50	41.6021 mg/l	growth inhibition	46 hrs	Tetrahymena pyriformis	Data is from DEPA
3	EC50	7.8 mg/l	no data	24 hrs	Nitrosomonas sp.	Data from publication for CAS 108-46-3
4	IC50	1600 mg/l	no data	96 hrs	methanogens	Data from publication for CAS 108-46-3

 

By applying weight of evidence approach to the studies summarized in the above table it can be observed that IC50 value is expected to be in 41.6021 –1600 mg/l and EC50 value was found to be in the range of 7.8 to 187.1003 mg/l in 12 to 96 hrs study