α,α-dichlorotoluene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1972

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Only mortality figures are given. No additional adverse effects are reported; therefore this study can be considered as reliable with restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

not specified

GLP compliance:

not specified

Test type:

acute toxic class method

Test material

Test material information

Constituent 1

Reference substance name:

α,α-dichlorotoluene

EC Number:

202-709-2

EC Name:

α,α-dichlorotoluene

Cas Number:

98-87-3

Molecular formula:

C7H6Cl2

IUPAC Name:

(dichloromethyl)benzene

Test animals

Species:

rat

Strain:

Sprague-Dawley

Remarks:

200-300 gram

Sex:

male

Details on test animals or test system and environmental conditions:

Also used 20-30 gram male CF1 (ICR derived) mice

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

Glass syringes with special needles were used to intubate all doses. The experimental animals were fasted for at least 16 hours prior to administration of the oral dose and were not given food for at least 2 hours after injection. This was to allow for more uniform absorption in all animals of the same species, since the amount of food in the stomach varies from animal to animal in the unfasted condition.
The intubation volume for both rats and mice was approximately 0.01 ml/gm body weight which meant that the average mouse received a volume of 0.25 ml, and the rat 2. 5 ml. The concentrations of the solutions or suspensions were adjusted to give the proper dose in the desired volume

Doses:

Initial rangefinding tests were conducted on each compound at the following dose levels: 5 mg/kg, 50 mg/kg, and 500 mg/kg. After the proper range had been found, subsequent doses were administered according to either a multiple of 2 or 1. 26 of the starting dose.

No. of animals per sex per dose:

5 rats and 5 mice were tested at each level chosen

Control animals:

not specified

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 2 462 mg/kg bw

Based on:

test mat.

95% CL:

> 1 788 - < 3 389

Mortality:

Data used to calculate LD50 in mg/kg bw (Mortality Response, N=5)
1000 (0) ; 2000 (1) ; 4000 (5)

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

The authors estimated the single oral dose toxicity of benzal chloride (CAS n° 98 -87-3) in male Sprague-dawley rats and in male CF-1 mice.
In the test conditions, the authors found a LD50 of 3250 and 2462 mg/kg bw respectively in male rats and mice. Therefore the substance is put in category 5 regarding the GHS criteria
Only mortality figures are given. No additional adverse effects are reported; therefore this study can be considered as reliable with restrictions.

Executive summary:

The authors estimated the single oral dose toxicity of benzal chloride (CAS n° 98 -87-3) in male Sprague-dawley rats and in male CF-1 mice.

In the test conditions, the authors found a LD50 of 3250 and 2462 mg/kg bw respectively in male rats and mice. Therefore the substance is put in category 5 regarding the GHS criteria

Only mortality figures are given. No additional adverse effects are reported; therefore this study can be considered as reliable with restrictions.