Registration Dossier
Registration Dossier
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EC number: 251-178-3 | CAS number: 32724-62-2
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Justification for type of information:
- Justification Document for the Category of Six Anthraquinone Dyes
LANXESS Deutschland GmbH has registered five mono-constituent anthraquinone dyes of similar chemical structure using a category approach: Solvent Violet 36 (CAS No 82-16-6), Solvent Green 3 (CAS No 128-80-3), Reinblau RLW (CAS No 41611-76-1), Reinblau BLW (CAS No 32724-62-2) and Solvent Green 28 (CAS No 4851-50-7). Additional data were taken from another registered anthraquinone dye, Solvent Blue 104 (CAS No 116-75-6), leading to a category consisting of six members (see attached justification in Category dossier). - Reason / purpose for cross-reference:
- read-across: supporting information
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study was performed on the source substance Reinblau RLW (CAS No. 41611-76-1), a member of the category. See the category approach justification document, attached in section 13, for further details. The test item showed 0 % respiration inhibition of activated sludge after a test period of 3 hours at the highest concentration of 1000 mg/L. Therefore an EC50 of higher than 1000 mg/L could be calculated for the test substance. The effect value relates to a nominal concentration, since no analytical monitoring was performed.
- Executive summary:
In order to test the toxicity to microorganism of the substance, activated sludge from predominantly domestic sewage plant was exposed to the test solution of three nominal concentrations of the substance (10, 100, 1000 mg/l), blank control solution and solution with positive reference substance for a period of 3 hours. Dissolved oxygen was recorded and respiration rates were calculated. 0 % respiration inhibition was observed at the highest concentration of test substance as water solution and therefore an EC50 (3h) < 1000 mg/L. The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.11 ”Activated sludge respiration inhibition” (2008). This test method is equal to OECD Guideline 209 (1984). This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microorganisms.
Reference
Description of key information
Read-across substance, Reinblau RLW (CAS No 41611-76-1); 3h-EC10 and 3h-EC50 (activated sludge) > 1000 mg/L (highest tested concentration); EU Method C.11; Currenta (2012).
To be confirmed with the new experimental study planned on the registered substance.
Key value for chemical safety assessment
Additional information
At the time being, this endpoint is covered by an experimental study performed on a source substance, Reinblau RLW (CAS No 41611-76-1), a member of the category. However, an OECD TG 209 study will be performed on the registered substance, Reinblau BLW. The dossier will be updated with the new study as soon at it becomes available.
In addition, similar results were obtained in the experimental studies on the toxicity to microorganisms on some other substances of the category: Solvent Violet 36 (CAS No 82-16-6) and Solvent Blue 104 (CAS No 116-75-6). See the category approach justification document, attached in section 13, for further details.
Global overview of the category approach for the endpoint:
Solvent Violet 36 | Solvent Green 3 | Reinblau RLW | Reinblau BLW | Solvent Blue 104# | Solvent Green 28 | |
Toxicity to microorganisms | 30min-EC50 (activated sludge) > 10000 mg/L (nominal)$ | Read across to Reinblau RLW
Other Lead Registrant data available but not considered key | 3h-EC10 and EC50 (activated sludge) > 1000 mg/L (nominal)$ | Study planned (OECD TG 209)
Read across to Reinblau RLW | 3h-EC50 and NOEC (activated sludge) > 1000 mg/L and ³ 1000 mg/L (nominal)$ | Study planned (OECD TG 209) |
# not registered by LANXESS, data consulted on ECHA disseminated website on April, 11 2022
$ experimental studies
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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