1,2-dichloro-4-nitrobenzene

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Principles of method if other than guideline:

Growth impairment of Tetrahymena pyriformis
Method following the protocol described by Schultz (1996). Tetrahymena in aquatic toxicology: QSARs and ecological hazard assessment. In: Proceedings of the International workshop on a protozoan test protocol with Tetrahymena in aquatic toxicity testing (Pauli & Berger, Eds.), pp 31-66.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Tetrahymena pyriformis

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

40 h

Test temperature:

27 +/- 1 °C

pH:

7.35

Details on test conditions:

Type: aquatic
TEST SYSTEM
- Test vessel: foam stoppered 250 mL Erlenmeyer flask
- No. of vessels per concentration (replicates): six to eight, range finding studies were performed previously
- No. of vessels per control (replicates): two
- the chemical is evaluated three times using freshly prepared stock solutions, each test was set up in duplicate


TEST MEDIUM
- media consists of distilled water, proteose peptone, glucose, yeast extract, TRIS-HCl, CaCl2, CuCl2, FeCl3, MgSO4, Fe(NH4)2(SO4)2, MnCl2, ZnCl2
- the media has to be sterilized prior to test begin



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
population density was measured spectrophotometrically at 540 nm

Duration:

40 h

Dose descriptor:

IC50

Effect conc.:

13.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Result is given in -log IGC50 = 1.16.
IG = Impairment of (population) growth
The result equals 0.069 mmol/L or 13.3 mg/L

Conclusions:

Toxicity to microorganisms test was done following the protocol described by Schultz (1996). Tetrahymena in aquatic toxicology. Tetrahymena pyriformis were exposed to test item for 40 hours. An IGC50 (inhibitory growth concentration) of 13.3 mg/L (nominal) was determined.

Executive summary:

Toxicity to microorganisms test was done following the protocol described by Schultz (1996). Tetrahymena in aquatic toxicology. Tetrahymena pyriformis were exposed to test item for 40 hours. An IGC50 (inhibitory growth concentration) of 13.3 mg/L (nominal) was determined.

Description of key information

Toxicity to microorganisms test was done following the protocol described by Schultz (1996). Tetrahymena in aquatic toxicology. Tetrahymena pyriformis were exposed to test item for 40 hours. An IGC50 (inhibitory growth concentration) of 13.3 mg/L (nominal) was determined.

Key value for chemical safety assessment

EC50 for microorganisms:

13.3 mg/L