1-bromo-3,4,5-trifluorobenzene

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the control and the 1.0, 3.2, 10, 32 and 100 mg/L test groups at 0 hours and the control and 1.0, 1.8, 3.2, 5.6. 1 0, 1 8, 32, 56 and 100 mg/l test groups at 48 hours (replicates R, and R: pooled) for quantitative analysis.

Vehicle:

no

Details on test solutions:

The test material (500 mg) was directly weighed into 5 litres (final volume) of reconstituted water and dispersed with the aid of ultrasonic disruption and shaking to prepare the 100 mg/L test concentration. This procedure was carried out as quickly as possible, the head space of the preparation vessel kept to a minimum and the vessel surrounded with dry ice in an order to lower the temperature as much as possible. These precautions were taken in an attempt to prevent losses of test material due to its suspected volatile nature. Dilutions were made from this 100 mg/L concentration in reconstituted water to prepare further test concentrations.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna (Straus)
- Source: Institut National de Recherche Chimique Appliquee, France

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

270 mg CaCO3/L

Test temperature:

21 °C

pH:

7.6 - 7.7

Dissolved oxygen:

7.9 - 8.6 mg/L

Nominal and measured concentrations:

Nominal concentrations: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L
Analytical measurements revealed that the test samples have been sufficiently stable.

Details on test conditions:

TEST SYSTEM
- Test vessel: 300 mL conical flasks
- Type: Closed. The test vessels were sealed with ground glass stoppers to reduce evaporation and possible losses of test material.
- Fill volume: 300 mL
- No. of organisms per vessel: 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark

EFFECT PARAMETERS MEASURED:
The number of immobilised Daphnia were recorded after 24 and 48 hours.

RANGE-FINDING STUDY
- Test concentrations: 0.10, 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: Immobilisation observed at 10 and 100 mg/L

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

5.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI 5.2 - 6.4 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI 2.9 - 3.8 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity to the freshwater invertebrate Daphnia magna has been investigated according to OECD Guideline 202 in a static test. The test substance concentration was monitored during the test. Based on measured concentrations, the 48 h-EC50 was determined to be 3.4 mg/L.

Executive summary:

The effects of the test item to Daphnia magna were determined in an acute immobilisation test according to OECD 202.

Following a preliminary range-finding study, twenty daphnids (2 replicates of 10 animals) were exposed to an aqueous dispersion of the test material at concentrations of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L for 48 hours under static test conditions. The numbers of immobilised daphnids were recorded after 24 and 48 hours.

The 48-hour EC50 for the test material to Daphnia magna based on nominal test concentrations was 4.8 mg/L with 95% confidence limits of 4.0 - 5.7 mg/L. The NOEC was 1.8 mg/L. Analysis of the test solutions throughout the study showed a marked decline in test concentrations and so it was considered justifiable to base the results on measured concentrations also. The 48-hour EC50, based on measured concentrations was 3.4 mg/L with 95% confidence limits of 2.9 - 3.8 mg/L. The NOEC was 1.6 mg/L.

 

Description of key information

The acute toxicity to the freshwater invertebrate Daphnia magna has been investigated according to OECD Guideline 202 in a static test. The test substance concentration was monitored during the test. Based on measured concentrations, the 48 h-EC50 was determined to be 3.4 mg/L (reference 6.1.3-1).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.4 mg/L

Additional information

The effects of the test item to Daphnia magna were determined in an acute immobilisation test according to OECD 202.

Following a preliminary range-finding study, twenty daphnids (2 replicates of 10 animals) were exposed to an aqueous dispersion of the test material at concentrations of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L for 48 hours under static test conditions. The numbers of immobilised daphnids were recorded after 24 and 48 hours.

The 48-hour EC50 for the test material to Daphnia magna based on nominal test concentrations was 4.8 mg/L with 95% confidence limits of 4.0 - 5.7 mg/L. The NOEC was 1.8 mg/L. Analysis of the test solutions throughout the study showed a marked decline in test concentrations and so it was considered justifiable to base the results on measured concentrations also. The 48-hour EC50, based on measured concentrations was 3.4 mg/L with 95% confidence limits of 2.9 - 3.8 mg/L. The NOEC was 1.6 mg/L (reference 6.1.3-1).