Dimethyl sulphate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessement.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Principles of method if other than guideline:

Method: other: BASF-Test

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl sulphate

EC Number:

201-058-1

EC Name:

Dimethyl sulphate

Cas Number:

77-78-1

Molecular formula:

C2H6O4S

IUPAC Name:

dimethyl sulfate

Details on test material:

- Name of test material (as cited in study report): dimethyl sulphate
- Physical state: liquid

Test animals

Species:

rat

Strain:

other: US

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: US rats
- Age at study initiation: young adult animals were used
- Diet: ad libitum
- Water: ad libitum
- Acclimation period:

ENVIRONMENTAL CONDITIONS
no data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: tragacanth (polysaccharide)

Details on oral exposure:

VEHICLE
- Concentration in vehicle: animals received emulsions of 0.5-20% test substance with tragacanth by oral gavage
- Amount of vehicle (if gavage): 25-1600 µl/kg bw

Doses:

25, 50, 64, 80, 100, 125, 200 and 1600 µl/kg body weight (corresponding to appr. 33.3, 66.5, 85.1, 106.4, 133.0, 166.3, 266.0 and 2128.0 mg/kg body weight assuming a density of 1.33 g/ml)

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: animals were observed and examined for clinical signs of toxicity at least daily
- Necropsy of survivors performed: yes; deceased animals and those sacrificed at the end of the observation period were necropsied
- Other examinations performed: clinical signs, body weight

Statistics:

LD50 was approximated

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 85.1 - < 133 mg/kg bw

Remarks on result:

other: 20/20 animals died in the 133.0 mg/kg body weight dose group, 9/20 (8 males) died in the 106.4 mg/kg body weight dose group and 3/20 (2 males) died in the 133.0 mg/kg body weight dose group.

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 106.4

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

> 85.1 - < 106.4

Effect levels 4

Sex:

male

Dose descriptor:

LD50

Effect level:

> 106.4 - < 133.2

Mortality:

First deaths were noted 1 h after dosing. All deaths occurred within 24 hours (see Table 1). No deaths occurred in the 50 µl/kg-group within the 7-day observation period.

Clinical signs:

Restlessness, irregular and accelerated breathing was reported for all dose levels. Dyspnoe, convulsions, apathy, hunched posture was observed at 100 µl/kg and higher doses.

Gross pathology:

Section findings, not discriminated according to dose level: Gastrectasy, decomposition, terminal lung oedema.

Any other information on results incl. tables

Table 1: Mortality

 

Dose level (µl/kg bw)	Mortality during the 7-day observation period
	Males	Females	Total
1600	10/10	10/10	20/20
200	10/10	10/10	20/20
125	6/10	10/10	16/20
100	10/10	10/10	20/20
80	1/10	8/10	9/20
64	1/10	2/10	3/20
50	0/10	0/10	0/20
25	0/10	0/10	0/20