2-Propanol, 1-amino-, compds. with polyethylene glycol hydrogen sulfate C12-14-alkyl ethers

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

27 SEP - 29 SEP 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: stock solution and all test concentrations at 0 and 48 h.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by adding 1.05 g test substance to 1 L deionised water. The stock solution was diluted with dionised water to give the respective test concentrations.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: clone 5
- Source: in-house culture
- Age at study initiation: < 24 h
- Method of breeding: for breeding, females are maintained in M4 medium in 1 L beakers. Fedd daily with Scenedesmus subspicatus.
- Feeding during test: none


ACCLIMATION
- Acclimation conditions (same as test or not): M4 medium in 1 L beakers
- Type and amount of food: the amount of food, comprising Scenedesmus subspicatus, is such that the amount added is consumed by the animals.
- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 +/- 1 °C

pH:

7.7 - 7.8

Dissolved oxygen:

7.9 - 8.1 mg/L

Nominal and measured concentrations:

Nominal test concentrations: 0.4, 0.7, 1.2, 2.0, 3.5, 6.0 and 10 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size: round-bottomed test tubes calibrated to 10 mL
- Aeration: none
- No. of organisms per vessel: 5 (+/-1)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water: 294 mg/L CaCl2 x 2H2O, 123 mg/L MgSO4 x 7 H2O, 63 mg/L NaHCO3, 5.5 mg/L KCl
- Ca/Mg ratio: 4 : 1 (2.5 mmol)


OTHER TEST CONDITIONS
- Photoperiod: continous darkness

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.7 mg/L

95% CI:

>= 6 - <= 10

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Results with reference substance (positive control):

Limit values afer 24 h: 1 mg/L 45% immobilised; 2 mg/L 100% immobilised

 Table 1: Immobilisation after 24 and 48 h.

Concentration [mg/L]	Test period (h)
	24 h	48 h
	Immobility [%]	Immobility [%]
Control	5	5
0.4	0	0
0.7^*	5	30
1.2	5	0
2.0	0	0
3.5	0	5
6.0	0	0
10	40	100

^*not used for evaluation, because no effect could be

observed in case the next following concentration

Table 2: Analytically determined test concentrations

Nominal concentration	0 h	48 h	Concentration for biological valuation [mg/L]
Control	0.07	--
0.4	0.37	0.38	0.4
0.7	0.73	0.70	0.7
1.2	1.2	1.2	1.2
2.0	1.9	2.0	2.0
3.5	3.2	3.4	3.5
6.0	5.5	5.9	6.0
10	9.1	9.7	10
Stock solution	955	--	1000

Table 3: Validity criteria for OECD 202 (2004)

Criterion from the guideline	Outcome	Validity criterion met
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.	5%	yes
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.	7.9 - 8.1 mg/L	yes

 

 

Validity criteria fulfilled:

yes

Remarks:

Please refer to table 3 in "Any other information on results incl. tables"

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

(e.g. non-GLP study; no analytical verification of the test concentrations)

Principles of method if other than guideline:

Method: other: Directive 79/831/EEC, Annex V, Part C

GLP compliance:

no

Analytical monitoring:

no

Details on test solutions:

Dilution water chemistry: according to guideline test water specifications

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus was originally supplied from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF.
Age: 6 - 24 hours old

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.7 ± 0.5 mmol/L

Test temperature:

292 - 294 K

pH:

8.02 to 10.30

Dissolved oxygen:

8.22 to 10.16 mg/l

Conductivity:

550 - 650 µSiemens/cm

Nominal and measured concentrations:

Nominal test concentrations: 500, 250, 125, 62.5, 31.2, 15.6, 7.8, 3.9 and 0 mg/L (control).

Details on test conditions:

Exposure vessels: Reagent tubes with flat bottom, 10 ml test volume
Light conditions: Diffuse light
Number of Replicates: Four Individuals /replicate: Five
Preparation of stock solution: Concentration of the substance in the stock solution was 500 mg/L.

Measurements:
a) pH values and dissolved oxygen: After 0 and 48 hours
b) Mobile daphnids: after 0, 3, 6, 24 and 48 hours

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

62.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

108.82 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

not neutralized

Reported statistics and error estimates:

The method of Spearman Kaerber was used to calculate EC 50 values after 6, 24 and 48 hours of exposure

Mobile daphnids

Nominal conc.(mg/l)	Mobile daphnids after
	0 h	3 h	6 h	24 h	48 h
0	20	20	20	20	20
3.9	20	20	20	20	20
7.8	20	20	20	20	20
15.6	20	20	20	20	20
31.2	20	20	20	20	20
62.5	20	20	20	20	20
125	20	20	20	20	6
250	20	20	20	0	0
500	20	1	0	0	0

pH values

Nominal conc.(mg/l)	pH values after
	0 h	48 h
0	8.03	8.15
3.99	8.32	8.03
7.81	8.54	8.02
15.6	8.89	8.05
31.2	9.20	8.14
62.5	9.53	8.4
125	9.79	8.71
250	10.04	8.96
500	10.3	9.09

Description of key information

EC50 (48 h): 7.7 mga.i./L (nominal, OECD 202)

Additional information

One GLP-study investigating the short-term toxicity of Alcohols, C12-14 (even numbered), ethoxylated (<=2.5 moles EO), sulfated, monoisopropanolamine salt to aquatic invertebrates is available. The study conducted according to OECD 202 (GLP) in a static system. The test organisms (Daphnia magna) were exposed to nominal substance concentrations of 0.4, 0.7, 1.2, 2.0, 3.5, 6.0 and 10 mg/L. The test concentrations were analytically verified using the MBAS assay (methylene blue active substances assay). The determined recovery rates were > 80%.

. After 48 h an EC50 value of 7.7 mg a.i./L (nominal) was determined.

 

A study available for monoisopropanolamine (MIPA) determined an EC50 (48 h) of 108.82 mg/L (nominal). The study was conducted according to Directive 79/831/EEC, Annex V, Part C without neutralisation of the test solutions.