Ethylamine

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian cell study: DNA damage and/or repair

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

The Testicular DNA-Synthesis Inhibition Test (DSI-Test)

Author:

Seiler J.

Year:

1981

Bibliographic source:

In: Stech M. und San R.: Short-Term Tests Chem.Carcinog., Heidelberg

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- male mice were used
- on the eve of test day: animals received injection of 18,5 kBq (0,5 µCi) (methyl-14C)-thymidine (subcuaneously in the back)
- the next day: prelabeled animals are randomly assigned to test and control groups (receive test substance i.p.)
- 3 hours later: again injection with labeled thymidine subcutaneously (10 µL = 0,37 MBq (10µCi) (methyl-14C)-thymidine
- 45 min later: i.p. injection of 3,0 mg unlabeled thymidine in 0,2 ml physiological saline (provides for a 5x10^4-fold dilution of the radioactive thymidine)
- 30 min later: mice were sacrified and their testes excised
- testes were transferred into 5% trichloroacetic acid (TCA), homogenized and centrifuged
- the pellet was washed twice in 5% TCA, resuspended in fresh 5% TCA, heated for 30 min at 95°C for DNA hydrolysis, cooled and centrifuged
- DNA content was determined photometrically
- radioactivity was determined by liquid scintillation counting

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Ethylamine

EC Number:

200-834-7

EC Name:

Ethylamine

Cas Number:

75-04-7

Molecular formula:

C2H5NH2

IUPAC Name:

ethanamine

Details on test material:

- Name of test material (as cited in study report): Ethylamine
- Analytical purity: no data

Test animals

Species:

mouse

Strain:

not specified

Sex:

male

Administration / exposure

Route of administration:

intraperitoneal

Duration of treatment / exposure:

3 hours

Frequency of treatment:

single application

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

5 mg/kg bw/day

Doses / concentrations 2

Dose / conc.:

15 mg/kg bw/day

Doses / concentrations 3

Dose / conc.:

50 mg/kg bw/day

No. of animals per sex per dose:

no data

Control animals:

yes

Examinations

Statistics:

nonparametric test (Wilcoxon-rank-test or equivalent)

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

not specified

Negative controls validity:

not specified

Positive controls validity:

not specified

Any other information on results incl. tables

Table 1: DSI Test Results of Ethylamine

Compound	Dose (mg/kg)	%DSI
Ethylamine	5 i.p.	0
	15	0
	50	5,6

Applicant's summary and conclusion

Conclusions:

Based on the DSI Test results of this publication, the test substance is a Noncarcinogen in mice.

Executive summary:

Male mice were treated with the test substance intraperitoneal. Three dose levels (5, 15, 50 mg/kb bw) were investigated. The results show that the test substance is a Noncarcinogen under the test conditions chosen.