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EC number: 202-424-3 | CAS number: 95-49-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: in accordance with the guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
- Principles of method if other than guideline:
- Single oral application by gavage of 1000, 2500, 2750, 3000, 3500, 4000 or 5000/kg bw (male rats) and 500, 1000, 2500, 3000, 4000 or 5000 mg/kg bw (female rats) in 0.5 ml lutrol/100g bw to 15 male and 15 female Wistar rats per dose. Observation period: 14 d, gross pathological examination, calculation of LD50 by Probit analysis.
Study design similar to OECD TG 401. - GLP compliance:
- no
- Test type:
- standard acute method
Test material
- Reference substance name:
- 2-chlorotoluene
- EC Number:
- 202-424-3
- EC Name:
- 2-chlorotoluene
- Cas Number:
- 95-49-8
- Molecular formula:
- C7H7Cl
- IUPAC Name:
- 1-chloro-2-methylbenzene
- Test material form:
- liquid
1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Doses:
- Male rats: 1000, 2500, 2750, 3000, 3500, 4000 or 5000 mg/kg bw
Female rats: 500, 1000, 2500, 3000, 4000 or 5000 mg/kg bw - No. of animals per sex per dose:
- 15
- Control animals:
- not specified
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 3 227 mg/kg bw
- Mortality:
- See table below
Any other information on results incl. tables
Mortality:
mg/kg bw | Male | mg/kg bw | Female |
1000 | 0/15 | 500 | 0/15 |
2500 | 0/15 | 1000 | 0/15 |
2750 | 3/15 | 2500 | 0/15 |
3000 | 5/15 | 3000 | 1/15 |
3500 | 10/15 | - | |
4000 | 14/15 | 4000 | 9/15 |
5000 | 15/15 | 5000 | 14/15 |
Mortality (male rats):
up to 2500 mg/kg bw no rat died
from 2750 mg/kg bw onward dose-related increase in mortality (2750 mg/kg bw: 3/15; 5000 mg/kg bw: 15/15)
Signs of intoxication from 2500 mg/kg bw onwards:
poor condition until the 10th day post application and for at least 3 days accompanied by sedation and difficulties in breathing and at higher doses by quasi-narcotic effects.
LD50 (male rats): 3227 (3060-3430) mg/kg bw
Mortality (female rats)
up to 2500 mg/kg bw no rat died
from 3000 mg/kg bw onward dose-related increase in mortality (3000 mg/kg bw: 1/15; 5000 mg/kg bw: 14/15)
Signs of intoxication from 1000 mg/kg bw onwards:
poor condition until the 10th day post application and for at least 3 days accompanied by sedation and difficulties in breathing and at higher doses by quasi-narcotic effects.
LD50 (female rats): 3860 (3542-4198) mg/kg bw
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Single oral application by gavage to male and female Wistar rats resulted in an LD50 (male rats) of 3227 mg/kg bw and an LD50 (female rats) of 3860 mg/kg bw.
- Executive summary:
In a reliable study the substance was applied once by gavage at doses of 1000, 2500, 2750, 3000, 3500, 4000 or 5000/kg bw (male rats) and 500, 1000, 2500, 3000, 4000 or 5000 mg/kg bw (female rats) in 0.5 ml lutrol/100g bw to 15 Wistar rats per sex and dose. The observation period was 14 days. Gross pathological examination was performed and the LD50 was calculated by Probit analysis.
The LD50 (male rats) was 3227 mg/kg bw, the LD50 (female rats) was 3860 mg/kg bw). At at necropsy 14 days post application a reduced size of abdominal organs, especially liver size was found. Mucus membrane of the stomach with focus of inflammation, stomach content appeared to be bloody.
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