α,2-dichlorotoluene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not applicable

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

α,2-dichlorotoluene

EC Number:

210-258-8

EC Name:

α,2-dichlorotoluene

Cas Number:

611-19-8

Molecular formula:

C7H6Cl2

IUPAC Name:

1-chloro-2-(chloromethyl)benzene

Details on test material:

Source: Occidental Chemical Corporation

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 180 - 300 g
- Fasting period before study: approximately 16 to 22 hours prior to the treatment
- Housing: individually in wire mesh cages
- Diet: ad libitum
- Water: ad libitum
- Other conditions were set according to AAALAC (Association for Assessment and Accreditation of Laboratory Animal Care) Standards

ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Doses:

280, 390, 540, 790 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Following administration, observations were made three times at the first day and daily thereafter for 14 days.
Any symptoms of toxicity of the animals were recorded.
Necropsy was made on all dead animals.
At 14 days all surviving animals are weighed, then they were sacrificed and necropsied.

Statistics:

LD50 with 95% confidence limits was calculated according to the method of C. S. Weil, Biometrics 249 (1952).

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

430 mL/kg bw

Based on:

test mat.

95% CL:

380 - 490

Mortality:

All male and female rats at 540 and 760 mg/kg bw, and three female at 390 mg/kg bw died at the first day.
Other rats were survived for 14 days.

Clinical signs:

other: Decreased motor activity and respiration, diarrhea, salivation and chromodacryorrhea

Gross pathology:

There were no gross tissue changes observable at the necropsy.

Other findings:

The oral  LD50 with 95% confidence limits was calculated to be 430 (380 - 490) mg/kg bw.

Any other information on results incl. tables

Table 1. Mortality of rats treated orally with OCBC
===================================================
                         Number of deaths
            ---------------------------------------
Dose        Hours   Days
(mg/kg)     0-4     1     2    3     4-14    Total
            M/F     M/F   M/F  M/F   M/F      M/F
--------------------------------------------------------
280         0/0     0/0   0/0  0/0   0/0      0/0
390         0/0     0/3   0/0  0/0   0/0      0/3
540         0/0     5/5   -/-  -/-   -/-      5/5
760         0/0     5/5   -/-  -/-   -/-      5/5
===================================================

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information R22: Harmful if swallowed / GHS Cat. 4 Criteria used for interpretation of results: EU

Conclusions:

o-Chlorobenzylchloride is considered to be harmful following acute oral exposure (LD50 430 mg/kg bw (95% confidence limits: 380-490 mg/kg bw)).

Executive summary:

In an acute oral toxicity study o-Chlorobenzylchloride was given in a single dose by gavage to five rats per sex at doses of 280, 390, 540, and 760 mg/kg bw; animals were observed for 14 days. Decreased motor activity and respiration, diarrhea, salivation and chromodacryorrhea were noticed within the observation period. All male and female rats at 540 and 760 mg/kg bw, and three female at 390 mg/kg bw died at the first day. Surviving animals were sacrificed and necropsied after 14 days. There were influence on body weight development and no gross tissue changes observable at necropsy. The oral LD50 with 95% confidence limits was calculated to be 430 (380 - 490) mg/kg bw.