4-chloro-2-nitroaniline

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well performed pre-guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Remarks:

pre-guideline study

Test material

Test material information

Constituent 1

Reference substance name:

4-chloro-2-nitroaniline

EC Number:

201-925-4

Cas Number:

89-63-4

Molecular formula:

C6H5ClN2O2

IUPAC Name:

4-chloro-2-nitroaniline

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Test substance was dissolved in ethanol und applicated into the test vessels. Ethanol concentration in the test vessels was below 1 mg/L. Test water of control group contains equal ethanol concentration.

Test organisms

Test organisms (species):

Idus idus

Details on test organisms:

- Common name: Golden Orfe (idus idus melanotus)
- Length at end of study: 4.9 - 6,1 cm (mean 5,6)
- Weight at study initiation: 2.2 - 3,3 g (mean 2,9 g)
- Food type: standard provender

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions: 20°C +/- 0,5 in test water

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

- total 17,4°dH
- carbonate 10°dH

Test temperature:

20°C +/- 0,5

pH:

8,5

Nominal and measured concentrations:

Study was performed with the concentrations 1 mg/L, 10 mg/L, 100 mg/L and 500 mg/L test item. Mortalities were recorded after 48 h and 96 h. Results see table below.

Details on test conditions:

- No. of organisms per vessel: 10
- Test vessel: glas aquaria (40 x 25 x 30 cm), filled with 20 L water
- Source/preparation of dilution water: dechlorinated tap water, activated coal filtered
- Aeration: 100 ml air per minute (> 7 mg/L O2)
- Photoperiod: 12/12
- Light intensity: 700 Lux

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 - < 100 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Animals died 7 min until 96 h after begin of study.
Dose groups >/= 10 mg/L showed the following behavioural abnormalities: heavy avoidance; enhanced sensitivity; supine position; disequilibrium; narcosis.
Died animals showed precipitation of test material at the body surface and the branchial arches.
The following signs of toxicity was observed after necropsy of dosing group 10 mg/L: body surface, content of gastrointenstinal tract, muscle tissue yellow discoloured. These signs did not occur with the control and the dosing group 1 mg/L.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Concentration [mg/L]	Mortality
	48 h	96 h
0	0 /10	0 /10
1	0 /10	0 /10
10	0 /10	2 / 10
100	10 / 10	10 / 10
500	10 / 10	10 / 10

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Classification for the environmental range is resulting from the more stringent data for short term toxicity to aquatic invertebrates, legal classification respectively.

Executive summary:

The 1 mg/L solution of the test item didn´t show any signs of toxicity nor lethality at all observation time points up to 96 hours. Therefore, the NOEC and LC₀were laid down as >= 1 mg/lL. The 10 mg/L solution resulted in 20 % mortality and the 100 mg/L solution in 100 % mortality of the fish . Therefore the LC₅₀(96) is > 10 mg/L - < 100 mg/L.