Cinnamaldehyde

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the OECD test guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

THe stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of Adams media and stock solution was analytically detected and then the test solutions were prepared for achieving the test concentrations of 0, 0.4, 0.8, 1.6, 3.2 and 6.4 mg/l, respectively.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water Flea
- Strain/clone: Straus
- Source: In-house breeding of Daphnia magna

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

155 mg/l as CaCO3

Test temperature:

18-22°C

pH:

7.4 (pH range 6 to 9)

Dissolved oxygen:

Above 3 mg/l

Nominal and measured concentrations:

Nominal concentrations: 0, 0.4, 0.8, 1.6, 3.2 and 6.4 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Material, size, headspace, fill volume: 100 mL glass beaker
- No. of organisms per vessel: 1 test organism
- No. of vessels per concentration (replicates): Duplicates
- No. of vessels per control (replicates): Duplicates

OTHER TEST CONDITIONS
- Photoperiod: 16 hour light and 8 hour dark
- Light intensity: 1000 – 1500 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Reproduction rate and the mobility behaviour / mortality rate of parent Daphnia was assessed at least three times a week.

TEST CONCENTRATION
- Test concentrations: Test chemical concentrations used for the study were 0, 0.5, 1, 2, 4 and 8 mg/L, respectively.

Reference substance (positive control):

no

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.402 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

No Immobility was found in the control beaker.

The mortality of the parent animals(female daphnia) does not exceed 20% at the end of the test

Validity criteria fulfilled:

yes

Conclusions:

Based on the reproduction of the test organism Daphnia magna due to the exposure of test chemical, the 21 days EC50 value was determined to be 0.402 mg/l (nominal concentration).

Executive summary:

Chronic toxicity to aquatic invertebrate study was conducted for 21 days for assessing the effect of test chemical on aquatic invertebrates. The study was performed following the OECD Guideline 211 (Daphnia magna Reproduction Test) under semi-static conditions. The stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of Adams media and stock solution was analytically detected and then the test solutions were prepared for achieving the test concentrations of 0, 0.4, 0.8, 1.6, 3.2 and 6.4 mg/l, respectively. The solution of test chemical was prepared by dissolving 200 mg of test chemical in 200 ml of Adams medium. Test chemical concentrations were not verified analytically. The remaining test solutions were prepared by dilution from the stock solution. Thus, test chemical concentrations used for the study were 0 (control), 0.4, 0.8, 1.6, 3.2 and 6.4 mg/L, respectively. Daphnia magna (water flea) was used as a test organism. Test organisms were exposed to five test chemical concentrations in 100 ml glass beaker. In addition to this, control test vessel was also setup during the study. Test vessels were then placed at temperature of 18 – 22°C, hardness 155 mg/l as CaCO3, pH 7.4 (Range 6 to 9 pH), dissolved oxygen greater than 3 mg/l and under a photoperiod of 16 hour light and 8 hour dark with 1000 – 1500 lux light intensity. Reproduction rate and the mobility behaviour / mortality rate of parent Daphnia was assessed. The mortality in the control groups did not exceeded 20% percent. Thus, the validity criterion of the test has been fulfilled. On the basis of the effect of test chemical on the reproduction of the test organism Daphnia magna, the 21 days EC50 value was determined to be 0.402 mg/l (nominal concentration). Thus, test chemical was considered as toxic to aquatic invertebrates and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.

Description of key information

Chronic toxicity to aquatic invertebrate study was conducted for 21 days for assessing the effect of test chemical on aquatic invertebrates (Experimental study report, 2019). The study was performed following the OECD Guideline 211 (Daphnia magna Reproduction Test) under semi-static conditions. The stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of Adams media and stock solution was analytically detected and then the test solutions were prepared for achieving the test concentrations of 0, 0.4, 0.8, 1.6, 3.2 and 6.4 mg/l, respectively. The solution of test chemical was prepared by dissolving 200 mg of test chemical in 200 ml of Adams medium. Test chemical concentrations were not verified analytically. The remaining test solutions were prepared by dilution from the stock solution. Thus, test chemical concentrations used for the study were 0 (control), 0.4, 0.8, 1.6, 3.2 and 6.4 mg/L, respectively. Daphnia magna (water flea) was used as a test organism. Test organisms were exposed to five test chemical concentrations in 100 ml glass beaker. In addition to this, control test vessel was also setup during the study. Test vessels were then placed at temperature of 18 – 22°C, hardness 155 mg/l as CaCO3, pH 7.4 (Range 6 to 9 pH), dissolved oxygen greater than 3 mg/l and under a photoperiod of 16 hour light and 8 hour dark with 1000 – 1500 lux light intensity. Reproduction rate and the mobility behaviour / mortality rate of parent Daphnia was assessed. The mortality in the control groups did not exceeded 20% percent. Thus, the validity criterion of the test has been fulfilled. On the basis of the effect of test chemical on the reproduction of the test organism Daphnia magna, the 21 days EC50 value was determined to be 0.402 mg/l (nominal concentration). Thus, test chemical was considered as toxic to aquatic invertebrates and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.4 mg/L

Additional information

Chronic toxicity to aquatic invertebrate study was conducted for 21 days for assessing the effect of test chemical on aquatic invertebrates (Experimental study report, 2019). The study was performed following the OECD Guideline 211 (Daphnia magna Reproduction Test) under semi-static conditions. The stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of Adams media and stock solution was analytically detected and then the test solutions were prepared for achieving the test concentrations of 0, 0.4, 0.8, 1.6, 3.2 and 6.4 mg/l, respectively. The solution of test chemical was prepared by dissolving 200 mg of test chemical in 200 ml of Adams medium. Test chemical concentrations were not verified analytically. The remaining test solutions were prepared by dilution from the stock solution. Thus, test chemical concentrations used for the study were 0 (control), 0.4, 0.8, 1.6, 3.2 and 6.4 mg/L, respectively. Daphnia magna (water flea) was used as a test organism. Test organisms were exposed to five test chemical concentrations in 100 ml glass beaker. In addition to this, control test vessel was also setup during the study. Test vessels were then placed at temperature of 18 – 22°C, hardness 155 mg/l as CaCO3, pH 7.4 (Range 6 to 9 pH), dissolved oxygen greater than 3 mg/l and under a photoperiod of 16 hour light and 8 hour dark with 1000 – 1500 lux light intensity. Reproduction rate and the mobility behaviour / mortality rate of parent Daphnia was assessed. The mortality in the control groups did not exceeded 20% percent. Thus, the validity criterion of the test has been fulfilled. On the basis of the effect of test chemical on the reproduction of the test organism Daphnia magna, the 21 days EC50 value was determined to be 0.402 mg/l (nominal concentration). Thus, test chemical was considered as toxic to aquatic invertebrates and hence, considered to be classified in 'aquatic chronic category 3' as per the CLP classification criteria.