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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented study report which meets basic scientific principles
Qualifier:
no guideline followed
Principles of method if other than guideline:
Method: other: fluorimetrically determined growth of algae exposed to dimethylethanolamine
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
66.08 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: recalculated
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
34.47 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: recalculated
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
24.49 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: recalculated
Conc.		Fluorimetric values		Control  
[mg/L]	inoculated	uninoculated	Diff.	[%]
-----------------------------------------------------------
1.562	2737		2		2735	86.83
3.125	2774		1		2773	88.03
6.25	3021		1		3020	95.87
12.5	2793		0		2793	88.67
25	2141		1		2140	67.94
50	689		1		688	21.84
100	178		0		178	5.65
100*	806		1		805	26.32 
0	3150		1		3149

*: neutralised sample
Validity criteria fulfilled:
yes
Conclusions:
EC10 = 24.49 mg/L,
EC50 = 66.08 mg/L
Executive summary:

The aquatic toxicity of dimethylaminoethanol towards algae was experimentally investigated (BASF AG, 1989). The growth of Desmodesmus subspicatus as test organism was fluorimetrically determined. The test organisms were exposed to test concentrations of 0 (control), 1.562, 3.125, 6.25, 12.5, 25, 50, 100 and 100 (pH adjusted) mg/L for 72 hours. As final result, an EC10 (72 h) of 24.49 mg/L and an EC50 (72 h) of 66.08 mg/L were determined.

Description of key information

The key values for chemical safety assessment are taken from the experimental result by BASF AG (1989).

Key value for chemical safety assessment

EC50 for freshwater algae:
66.08 mg/L
EC50 for marine water algae:
40 mg/L
EC10 or NOEC for freshwater algae:
24.49 mg/L

Additional information

The aquatic toxicity of dimethylaminoethanol (DMAE) towards algae was experimentally investigated (BASF AG, 1989). The growth of Desmodesmus subspicatus as test organism was fluorimetrically determined. The test organisms were exposed to test concentrations of 0 (control), 1.562, 3.125, 6.25, 12.5, 25, 50, 100 and 100 (pH adjusted) mg/L for 72 hours. As final result, an EC10 (72 h) of 24.49 mg/L and an EC50 (72 h) of 66.08 mg/L were determined.

In the supporting study - a static screening study of Eide-Haugmo et al. (2012) with marine phytoplankton species Skeletonema costatum - aquatic toxicity of dimethylethanolamine (DMAE, CAS 108 -01 -0) and further 42 amines was determined. The test was performed according to ISO guideline 10253 of 1999, natural seawater served as test medium. Test substance concentrations used in this study were based on pre-tests with wide concentration-ranges. For DMAE a EC50 (72 h) value of 40 mg/L was calculated.