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Ecotoxicological information

Long-term toxicity to fish

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Description of key information

Most sensitive long term toxicity data for 3 different freshwater fish species are summarized in the EU-RAR (2008). According to these data, the effect concentrations for Nickel hydrogencitrate were calculated:
Brachydanio rerio: no effect concentration (8d, hatchability) = 0.13 mg Trinickel dicitrate/L
Pimephales promelas: NOEC (32d, growth) = 0.180 mg Trinickel dicitrate/L
Oncorhynchus mykiss: NOEC (8d, growth) = 0.422 mg Trinickel dicitrate/L (most sensitive data for this species)
Most sensitive long term toxicity data for 2 different marine fish species (as cited in EU-RAR, 2008)
Atherinops affinis: EC10 (40d, survival) = 11.326 mg Trinickel dicitrate/L
Cyprinodon variegatus: EC10 (28d, growth) = 65.331 mg Trinickel dicitrate/L

Key value for chemical safety assessment

Additional information

Accepted data on chronic single-species toxicity tests resulting in high quality NOEC/L(E)C10 values (expressed as Ni) for freshwater fish (n = 21) are summarised in the EU-RAR, 2008. Chronic data for 3 different fish species were extracted and used in the effects assessment. The NOEC/L(E)C10 values for fish range from 40 μg Ni/L (0.13 mg Trinickel dicitrate/L) for Brachydanio rerio to 1,100 μg Ni/L (3.462 mg Trinickel dicitrate/L) for Oncorhynchus mykiss. The most sensitive data for Brachydanio rerio are shown in detail in the key study record of chap 6.1.2, further results for other freshwater fish are attached in this key study record.


[citric acid, nickel salt] is present on the surface of an inert carrier material (aluminium oxide). Depending on the pH value to prepare the impregnation solution or present while impregnation different molar ratios between nickel and citric acid are possible.

For this reason the concentrations of the single Nickel constituents of [citric acid, nickel salt] are various. The calculated values refer to the constituent Trinickel dicitrate and have to seen as a lower threshold value.

Accepted data on chronic single-species toxicity tests resulting in accepted high quality reliable NOEC or L(E)C10 values (expressed as Ni) for marine fish are summarized in the EU-RAR, 2008, too. EC10 values were in a range from 3599 μg Ni/L (11.326 mg Trinickel dicitrate/L) for survival of the topsmelt, Atherinops affinis, to 20760 μg Ni/L (65,331 mg Trinickel dicitrate/L) for growth of the sheepshead minnow, Cyprinodon variegatus. The results for the marine species are only shown in the attachment of the key study record of chapter 6.1.2.