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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Description of key information

LD50 cut-off = 370 mg/kg bw Trinickel dicitrate
LD50 > 1480 mg/kg bw Trinickel dicitrate

Key value for chemical safety assessment

Acute toxicity: via dermal route

Endpoint conclusion
mg/kg bw

Additional information


The oral acute toxicity study has been performed according to OECD guideline 423. First, groups of 6 female Sprague Dawley rats received a single oral dose of 2000 mg/kg bw nickel hydrogencitrate (recalculated for Trinickel dicitrate: 1480). All animals died within 72 hours after dosing. Mortalities were preceded by a decrease in spontaneous activity associated with partial ptosis, piloerection and bradypnea as well as a decrease in muscle tone and righting reflex. Furthermore, increased lachrymation was observed. In a second step, 6 female rats of the same strain were dosed with 300 mg/kg bw nickel hydrogencitrate. No animal died. At this dose level, there were no effects related to treatment. The LD50 cut-off was determined as 500 mg/kg bw nickel hydrogencitrate. (recalculated for Trinickel dicitrate: 370)


No reliable acute study public available.



For the dermal route, a study according to OECD guideline 402 has been performed with nickel hydrogencitrate in 5 Sprague Dawley rats of each sex at a single dose of 2000 mg/kg bw. No mortality occured. There were no treatment-related effects. Therefore, the LD50 was determined as higher than 2000 mg/kg bw (recalculated for Trinickel dicitrate: 1480).


Nickel hydrogencitrate and Trinickel dicitrate are comparable: Since citric acid is a multifunctional acid, at different pH values various intermediate ions like citrate, hydrogencitrate and dihydrogencitrate are formed.

[citric acid, nickel salt] is present on the surface of an inert carrier material (aluminium oxide). Depending on the pH value to prepare the impregnation solution or present while impregnation different molar ratios between nickel and citric acid are possible.

For this reason the concentrations of the single Nickel constituents of [citric acid, nickel salt] are various. The calculated values refer to the constituent Trinickel dicitrate and have to seen as a lower threshold value.

Justification for classification or non-classification

Based on the available data, the substance [citric acid, nickel salt] needs to be cassified:


EU: R22 Harmful if swallowed.

CLP: Category 4


There is conclusive data available which is not sufficient for classification.


There is conclusive data available which is not sufficient for classification.