1,1',1''-nitrilotripropan-2-ol

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Study period:

08. Mar 1966 - 21. Mar 1966

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented study report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1966

Report date:

1966

Materials and methods

Principles of method if other than guideline:

other: BASF-Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,1',1''-nitrilotripropan-2-ol

EC Number:

204-528-4

EC Name:

1,1',1''-nitrilotripropan-2-ol

Cas Number:

122-20-3

Molecular formula:

C9H21NO3

IUPAC Name:

1-[bis(2-hydroxypropyl)amino]propan-2-ol

Test material form:

solid

Specific details on test material used for the study:

- Analytical purity: ca. 92-95 %
- Impurities (identity and concentrations): ca. 3-6 % Diisopropanolamine; < 1 % Monoisopropanolamine

Test animals

Species:

mouse

Strain:

other: Kisslegg-mice

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Doses:

200, 400, 800, 1000, 1250, 1600 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 1 400 mg/kg bw

Mortality:

1600 mg/kg: all animals died within the first 24 h.
1250 mg/kg: 1 female died within 24 h.

Clinical signs:

1600 mg/kg:
15 min post application: abdominal position, fluffed fur, intermittent respiration.
25 min post application: slight tremulous convulsions, abdominal position, accelerated respiration, mouth discharge, partly dyspnoea.

1250 and 1000 mg/kg:
15 min post application: staggering, accelerated respiration, abdominal position, flattened ears.
1 and 2 days post application: heavy ruffled fur, accelerated respiration, high stepping tremulous gait.
3 and 4 days post application: accelerated respiration, high stepping tremulous gait.

800 and 400 mg/kg:
10 min post application: high stepping, hoppling gait, accelerated respiration, gaunted sides, flattened ears, animals don´t continue in abominal position.
5 h and 1 day post application: abdominal position, heavy fluffed fur, gaunted sides, very calm attitude.

200 mg/kg: 5 h post application fluffed fur and accelerated respiration.

Gross pathology:

1250 mg/kg: 6x agglutination of intestinal loops.
800 mg/kg: 2x agglutination of intestinal loops.

Any other information on results incl. tables

Mortality:

Dose (mg/kg)	Conc. (%)	1 h	24 h	48 h	7 days	14 days
1600	20	0/10	10/10	10/10	10/10	-
1250	8	0/10	1/10	1/10	1/10	-
1000	8	0/10	0/10	0/10	0/10	-
800	8	0/10	0/10	0/10	0/10	-
400	8	0/10	0/10	0/10	0/10	-
200	2	0/10	0/10	0/10	0/10	-