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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Link to relevant study record(s)

Description of key information

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable but failing 10-day window

Additional information

The test substance, Carbolic oil, was tested for the ready biological degradability in Closed Bottle Test.

Test performance

The test was performed according to: Method C.4E - Closed Bottle Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.

The results of biological degradation are related to experimentally determined COD values of test and reference substance at the beginning of the test.

Owing to the limited water solubility of test substance dosing the dispersion in mineral medium into each bottle was implemented.

Sodium benzoate was used as the reference substance. The dosage was carried out from the stock solution.

COD of the test substance in medium at the beginning of the main test:           3.38 mg.L-1

COD of reference substance in medium at the beginning of the main test: 3.44 mg.L-1

In parallel to the main test the toxicity test was performed.

The test substance has not available molecular formula, it can contain of nitrogen in the chemical composition. The oxidized nitrogen forms were determined and the correction for nitrification was carried out.

The test was performed at temperature of 20 ± 1 °C with the pH values of solutions 7-8 at the beginning of the test.

Validity of test

The prescribed validity criteria in the test were fulfilled. The test substance was not inhibiting for the used inoculum. Since all criteria of acceptability were met, this study is considered to be valid.

Test results

In this 28-day study of ready biological degradability the degradation of 59.9 % of the test substance, Carbolic oil, was attained in the end of study .