2,4,6-trichloro-1,3,5-triazine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-08-19 to 1991-09-23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

adopted: 1981-02-02

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-trichloro-1,3,5-triazine

EC Number:

203-614-9

EC Name:

2,4,6-trichloro-1,3,5-triazine

Cas Number:

108-77-0

Molecular formula:

C3Cl3N3

IUPAC Name:

trichloro-1,3,5-triazine

Constituent 2

Reference substance name:

cyanuric chloride

IUPAC Name:

cyanuric chloride

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Versuchstierzucht Winkelmann, Borchen, Kreis Paderborn, Germany
- Age at study initiation: 2 to 3 months
- Weight at study initiation: 170 - 210 g
- Housing: 5 animals per cage (Makrolon type III)
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 °C +- 2
- Humidity (%): ca. 50 %
- Air changes (per hr): ca. 10
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose/head only

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: inhalation chamber (TSE Labortechnik, Bad Homburg)
- Exposure chamber volume: 20 L
- Method of conditioning air: VIA-Drucklufttrockner
- System of generating particulates/aerosols: Wright-Dust-Feeder + self constructed metal cyclone as particle precipitator (used at higher doses)
- Method of particle size determination: Berner- or Andersen-cascade impactor
- Treatment of exhaust air: aerosol filter (absorbent cotton) and + combustor
- Temperature, humidity, pressure in air chamber: 24 °C, 25 %


TEST ATMOSPHERE
- Brief description of analytical method used: GC
- Samples taken from breathing zone: yes

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: see "any other information on materials and methods including tables"
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): see "any other information on materials and methods including tables"
- stability of test atmosphere: total carbon analyzer (Ratfisch RS 55)

Analytical verification of test atmosphere concentrations:

yes

Remarks:

see (I)

Duration of exposure:

4 h

Concentrations:

- 37.6, 150.6, 177.3, 289.3, 449.1 mg/m³, (respective contribution to the concentrations by particles: 0.0, 2.3, 18.8, 44.7, 165.5 mg/m³)

No. of animals per sex per dose:

- 5

Control animals:

other: animals of the study were compared to periodically tested animals (once per 3 month, clean air)

Details on study design:

- Duration of observation period following administration: 28 days
- Frequency of observations: several times on day 0, daily thereafter; frequency of weighing: day 1, day 3, day 7, day 14 and day 28
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, gross necropsy

Statistics:

Maximum-Likelihood where applicable

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

170 mg/m³ air

Based on:

test mat.

95% CL:

137 - 213

Exp. duration:

4 h

Effect levels 2

Sex:

male

Dose descriptor:

LC50

Effect level:

150 mg/m³ air

Based on:

test mat.

Exp. duration:

4 h

Remarks on result:

other: this value was approximated, as the slope of dose response curve was very steep

Effect levels 3

Sex:

female

Dose descriptor:

LC50

Effect level:

201 mg/m³ air

Based on:

test mat.

95% CL:

157.7 - 257.9

Exp. duration:

4 h

Mortality:

- see table on mortality, body weight decrease, clinical signs and necroscopy
- mortality occurred between day 1 and day 14 post application

Clinical signs:

other: - clinical signs reported for days 0,1,2,3,7,14,21,28 - see table on mortality, body weight decrease, clinical signs and necroscopy

Body weight:

- see table on mortality, body weight decrease, clinical signs and necroscopy

Gross pathology:

- see table on mortality, body weight decrease, clinical signs and necroscopy

Other findings:

- reflexes: see table on mortality, body weight decrease, clinical signs and necroscopy

Applicant's summary and conclusion

Interpretation of results:

Category 2 based on GHS criteria

Remarks:

Migrated information

Conclusions:

Under the conditions of this acute inhalation toxicity study a combined LC50 for both sexes of 170 mg/m³ air (137 — 213, 95% CL) and a LD50 for males of 150 mg/m³ air were deduced for rats.