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EC number: 292-607-4 | CAS number: 90640-86-1 Distillate from the fractional distillation of coal tar of bituminous coal, with boiling range of 240°C to 400°C (464°F to 752°F). Composed primarily of tri- and polynuclear hydrocarbons and heterocyclic compounds.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Creosote
- EC Number:
- 232-287-5
- EC Name:
- Creosote
- Cas Number:
- 8001-58-9
- IUPAC Name:
- 8001-58-9
- Details on test material:
- - Name of test material (as cited in study report): Creosote
Creosote WEI-Type B, containing less than 50 ppm BaP
- Substance type: organic
- Physical state: liquid
- Composition
Name CAS-No. Concentration [w%]
RUETGERS Chemicals // Biochem. Inst.Grimmer
=================================================
Naphthalene 91-20-3 5.07 5.39
Fluorene 86-73-7 3.06 3.07
Acenaphthylene 208-96-8 -- 0.09
Acenaphthene 83-32-9 4.09 4.04
Phenanthrene 85-01-8 8.95 8.56
Anthracene 120-12-7 0.45 0.38
Fluoranthene 206-44-0 3.70 3.84
Pyrene 129-00-0 2.49 2.08
Benz[a]anthracene 56-55-3 0.04 0.04
Chrysene 218-01-9 0.03 0.03
Benzo(a)pyrene 50-32-8 <10 ppm ca. 6. ppm
----------------------------------------------------------------
Total of 5 remaining EPA-PAH: --- < 20 pp
----------------------------------------------------------------
(Benzo[b]fluoranthene 205-99-2
Benzo[k]fluoranthene 207-08-9
Indeno[1,2,3-cd]pyrene 193-39-5
Dibenzo[a,h]anthracene 53-70-3
Benzo[ghi]perylene) 191-24-2
===========================================
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- --
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct addition and agitation by aeration in the presence of inoculum
- Differential loading: yes
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Cultivation: Activated sludge from the effluent of the aeration tank of the municipal WWTP at Taunusstein-Biederstadt/Germany.
The isolated sludge mixture (MLSS) (approx. 4 g/l dry mass) was pre-incubated in synthetic medium for 2 d.
The culture medium contained 8 ml synthetic sewage feed (prepared from peptone (16 g/l), beef extract (11 g/l), urea (3 g/l),
mineral salts), 100 ml inoculum and tap water for a total volume of 250 ml.
- Initial biomass concentration: approx. 1.5 g/l (dry mass).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- = incubation time of the test solutions (test substance + inoculum) under aeration prior to measuring
- Post exposure observation period:
- --
Test conditions
- Hardness:
- --
- Test temperature:
- 18.0 - 18.1 °C
- pH:
- 8.3 - 8.4
- Dissolved oxygen:
- parameter to be measured
- Salinity:
- --
- Nominal and measured concentrations:
- 125, 250, 500, 1000, and 2000 mg/l (nominal, without separation of undissolved material) (derived from a pre-test).
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed at measuring of O2 consumption
- Material, size, headspace, fill volume: 250-ml glass bottle with inlet for aeration
- Aeration: 3 h until start of determination of the respiration rate
- Measuring equipment: pH-Electrode: WTW portable; O2-Electrode WTW OXI 530
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): Two parallel trials without test substance / one abiotic control containing 2000 mg creosote/L
(without biomass)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : O2-consumption
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5- dichlorophenol (5 - 30 mg/L)
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- other: inhibitory loading EL50
- Effect conc.:
- 670 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% C.I of the inhibition rate: approx. +- 12 %
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: dispersion
- Effect concentrations exceeding solubility of substance in test medium: yes,
(At the EL50, the WAF is estimated to have been between 30 and 40 mg dissolved carbon/L, based on water solubility data.)
- Pre-test:
Test loadings: 10, 100, and 1000 mg/L
Results: No inhibition, but about >40-% stimulation (10 mg/L);
26-% inhibition (100 mg/L);
50-% inhibition (1000 mg/L) - Results with reference substance (positive control):
- - Results with reference substance valid? yes, at 5, 15, 30 mg/L
- Relevant effect levels: EC50 approx. 10 mg/L
Any other information on results incl. tables
Summary of effects data: Inhibition in % versus loadings of test substance:
Test [mg/l, nominal] |
Respiration Rate |
Inhibition of Respiration [%] |
Control |
0.84 |
0 |
Abiotic Control [2000 mg/l] |
0 |
-- |
125 |
0.71 |
18.9 |
250 |
0.54 |
38.4 |
500 |
0.46 |
47.4 |
1000 |
0.48 |
45.1 |
2000 |
0.22 |
74.9 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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