Fatty acids, C16-18 and C18-unsatd., reaction products with triethanolamine, di-Me sulfate-quaternized

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH

1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that source and target substances have similar (eco)toxicological properties because
• they share structural similarities with common functional groups: One quaternised ethanolamine moiety, one to three, mainly two ester groups with a typical UVCB distribution with long-chain fatty acids of natural origin. The molecular structure is almost identical.
• they are manufactured from similar resp. identical precursors (triethanolamine, long-chain fatty acids, dimethyl sulphate) under similar conditions. Therefore common breakdown products via physical and biological processes, which result in structurally similar chemicals are evident
• A constant pattern in the changing of the potency of the properties across the TEA-Esterquats by chain-length and the grade of esterification is not observed, because the fatty acid chain-length distribution is too narrow and similar and the distribution of mono-, di-, and tri-esters is identical. Some variation caused by variation in C=C double bonds may occur and will be discussed at the relevant endpoint.

2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
See justification for read-across attached to chapter 13 of this IUCLID file.

3. ANALOGUE APPROACH JUSTIFICATION
See justification for read-across attached to chapter 13 of this IUCLID file.

4. DATA MATRIX
See justification for read-across attached to chapter 13 of this IUCLID file.

Reason / purpose for cross-reference:

read-across: supporting information

Reason / purpose for cross-reference:

read-across source

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

60 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Description of key information

30 min EC10 = 29.6 mg a.i./L; 30 min EC50 = 60 mg a.i./L (DIN 38412, part 27); GLP, RL1; read-across: fully saturated TEA-Esterquat

Key value for chemical safety assessment

EC50 for microorganisms:

60 mg/L

EC10 or NOEC for microorganisms:

29.6 mg/L

Additional information

Reliable and relevant data are available for partially unsaturated TEA-Esterquat and the structurally related source substance fully saturated TEA-Esterquat. A justification for read-across is attached to Iuclid section 13.

 

In a study with fully saturated TEA-Esterquat the acute bacterial toxicity towards Pseudomonas putida was determined under static conditions according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest) at test material concentrations of 10, 30, 100 mg test mat./L. The calculated effect concentrations after 30 minutes were as follows: EC0: 24.0 mg a.i./L, EC10: 29.6 mg a.i./L, EC50: 60 mg a.i./L.

 

Supporting data are available for partially unsaturated TEA-Esterquat:

In a 3 hour toxicity study conducted according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test) and EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test), the cultures of activated sludge of a predominantly domestic sewage treatment plant were exposed to partially unsaturated TEA-Esterquat (CAS No. 157905-74-3) at nominal concentrations of 0, 1, 3, 9, 27, 81, 243 mg/L under static conditions. There was no inhibitory effect of the test item at any concentration; the deviations from the untreated controls were less than 15 %, both after 30 min and 3 hours. The 3 h EC50 value based on inhibition of respiration was > 243 mg/L.

 

The toxic effect of partially unsaturated TEA-Esterquat on Pseudomonas putida was assessed by measuring the respiration rate under defined conditions in the presence of different concentrations of partially unsaturated TEA-Esterquat (solvent free, 100 % a.i.) according to DIN 38412, part 27, 1992 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest)) at nominal concentrations of control, 10, 30 100, 300 1000, 3000, 10000 mg a.i./L. The calculated effect concentrations were as follows: 30 min EC0: 30 mg/L, 30 min EC10: 35 mg/L, and 30 min EC50 110 mg/L.

 

The toxic effect of partially unsaturated TEA-Esterquat on activated sludge from a biological treatment plant was assessed by measuring the respiration rate under defined conditions in the presence of different concentrations of the test article. According to OECD guideline 209 a concentration range between 16 and 125.0 mg/L of the test article was used in the test. Concentrations of 250 mg/L and 125 mg/L supported the respiration rates. Values of 2.6 % and 17.9 % compared to the untreated controls were obtained. Inhibition of the respiration rates were obtained with concentrations of 62, 32, and 16 mg/L of the test article and were determined to be 2.6 %, 7.7 %, and 7.7 %, respectively, compared to the untreated control. From the results, it was concluded to state that the test article did not severely inhibit the respiration rate of activated sludge obtained from a biological treatment plant. The stock solution of the test substance was prepared immediately before the test. The EC50 of the test substance is higher than 250 mg/L.