Zirconium zircon with encapsulated cadmium selenium sulphide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Only nominal concentration given; no data about measured test substance concentration is given (in eluats); only a brief description of the test conditions is given.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 17.07.92

Deviations:

yes

Remarks:

Only nominal concentration given.

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Tests were performed with the eluates of the following nominal test substance suspension concentrations (dissolved fraction of the test substance, filtered after 24 h stirring of each respective test concentration): 100, 1000 and 10000 mg/L (screening test), 1000, 2000, 4000, 8000 and 10000 mg/L (Main test), 10000 mg/L (additional limit test).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Test temperature:

22.0 - 23.0 °C (10000 mg/L)

pH:

Control: 8.40 - 8.33
Main test (10000 mg/L): 7.93 - 8.22

Dissolved oxygen:

Control: 8.01 - 8.30 mgO2/L
Main test (10000 mg/L): 7.83 - 8.19 mg O2/L

Nominal and measured concentrations:

Nominal concentrations: 1000, 2000, 4000, 8000, 10000 mg pigment/L

Details on test conditions:

Number of fishes in main test: 7 per tank (3L)
Light: 16h photoperiod daily

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

>= 10 000 other: mg/L dissolved fraction

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

>= 10 000 other: mg/L dissolved fraction

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

>= 10 000 other: mg/L dissolved fraction

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 10 000 other: mg/L dissolved fraction

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 10 000 other: mg/L dissolved fraction

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

The LC50 values were calculated by linear regression.

Validity criteria fulfilled:

not specified

Conclusions:

Study according to the guideline. Acute toxic effects could not be observed; the 96h-LC50 for silicic acid, zirconium salt, cadmium pigment encapsulated toxicity (survival) to Brachydanio rerio is >10,000 mg/L (dissolved fraction at a loading of 10,000 mg/L after 24 hours of stirring). This value will further be used in the chemical safety assessment. The corresponding LC0 is ≥ 10,000 mg/L (dissolved fraction at a loading of 10,000 mg/L after 24 hours of stirring) as well.

Description of key information

The results of a key study with Zirconium zircon with encapsulated cadmium selenium sulphide indicate a low potential for acute toxicity to fish. The respective 96h LC50 of Zirconium zircon with encapsulated cadmium selenium sulphide on freshwater fish (Danio rerio), obtained in a test according to OECD 203, is > 10,000 mg/L (dissolved fraction at a loading of 10,000 mg Zirconium zircon with encapsulated cadmium selenium sulphide/L after 24 h stirring). The corresponding 96h LC0 for acute effects of Zirconium zircon with encapsulated cadmium selenium sulphide was also estimated to be > 10,000 mg/L (Weyand, 2001). Nevertheless, read-across to the assessment entity soluble cadmium substances is applied since cadmium cations determine fate and toxicity in the environment.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Remarks:

dissolved fraction at a loading of 10,000 mg/L after 24 h stirring

Effect concentration:

> 10 000 mg/L

Additional information

One reliable study (Klimisch 2) is availbale for the determination of acute toxicity of the pigment Zirconium zircon with encapsulated cadmium selenium sulphide is available. Freshwater 96h-LC50 and LC0 for the acute effect of the substance on Brachydanio rerio were determined to be >10000 mg/L (Weyand, 2001).