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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Description of key information

No effect on long-term toxicity to aquatic invertebrates up to the limit of water solubility.

Key value for chemical safety assessment

Additional information

No long-term studies of Fatty acids, C16-18 and C18-unsatd., 2-ethylhexyl esters (CAS 85049-37-2) on toxicity to invertebrates are available. The assessment was therefore based on studies conducted with the category members 2-ethylhexyl oleate (CAS 26399-02-0) and Fatty acids, C8-16, 2-ethylhexyl esters (CAS 135800-37-2), as part of a read across approach, which is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5. Grouping of substance and read across approach. Further justification is given within the endpoint summary 6.1 and within the category justification section 13. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was entered into IUCLID. Nevertheless, as can be seen in the data matrix of the category justification in Section 13, all reliable data in the category support the hazard assessment for this endpoint, by showing a consistent pattern of results.

The study with 2-ethylhexyl oleate (CAS 26399-02-0) investigated the long-term effects to the freshwater invertebrate Daphnia magna (Kuech 1995). The study was performed according to the OECD guideline 202, part 2 of 1993. Test concentrations of nominal 1 and 100 mg/L were appplied as filtered test solutions. Analytically, concentrations of <0.01 mg/L were retrieved from both nominal concentrations. Effects to Daphnia magna were observed after 21 days with no effect at 1 mg/L and 90% mortality (on day 19) at 100 mg/L. The authors stated, that the deaths in the higher concentration could be attributed to sample contamination. Therefore, a NOEC(21 d, mortality) >1 mg/L (nominal) was reported. Similarly, 23% reduction of reproduction (number of offspring) in the 100 mg/L sample occurred after 21 days, with no effect in the 1 mg/L sample resulting in a NOEC(21 d, reproduction) > 1 mg/L. If the reproduction up to day 17 in the 100 mg/L sample was compared to the reproduction in the control, no reduction of reproduction was stated.

In the study conducted with Fatty acids, C8-16, 2-ethylhexyl esters (Wierich 1998), 1 and 100 mg/L test substance were both tested as filtered test solutions. In the filtered solution of 1 mg/L no effects on Daphnia magna were observed for mortality and reproduction, on the other hand, in the filtered solution of 100 mg/L a significant reduction of the reproduction was stated. The authors supposed that the effects in the 100 mg/L filtered test solution are due to a better water soluble by-compound (for example a short chain fatty acid ester) or - less likely due to a toxic impurity. Therefore, a NOEC(21d, reproduction) of > 1 mg/L (nominal) was given.

Thus, based on the above mentioned result, and due to the structural and profile similarities of the substances, as are explained with in the overall endpoint summary 6.1 it can be concluded that no toxicological effects on aquatic invertebrates are expected up to the limit of water solubility for Fatty acids, C16 -18 and C18 -unsatd., 2 -ethylhexyl esters (CAS 85049 -37 -2).