Tetrabutylammonium bromide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17-07-2018 to 21-07-2018

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L,respectively. Potable water (passed through reverse osmosis system) was used.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Source: Anytime pet, Jaripatka, Nagpur.
- Length at study initiation (length definition, mean, range and SD): average 2.04 cm
- Weight at study initiation (mean and range, SD): average 0.085 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

24, 48, 72 and 96 hrs

Hardness:

181.5 mg of CaCO3

Test temperature:

23°C

pH:

7.2

Dissolved oxygen:

7.0 mg/l

Nominal and measured concentrations:

6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L, and 100 mg/L nominal concentrations were used

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: Plastic aquaria having the water capacity of 5 liters filled witrh 2 liters of water.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No effect were observed

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Other details not known

Details on results:

After the exposure of test chemical for 96 hrs with thetest aqnimal zebra fish, no effect were observed at 100 mg/l.

Sublethal observations / clinical signs:

Table: Mortality and symptoms

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3hr			6hr		24hr			48hr		72hr			96hr
			V1	V2		V1	V2	V1	V2		V1	V2	V1	V2		V1	V2
Control	4	4	-	-		-	-	-	-		-	-	-	-		-	-
6.25	4	4	-		-	-	-	-		-	-	-	-		-	-		-
12.5	4	4	-		-	-	-	-		-	-	-	-		-	-		-
25	4	4	-		-	-	-	-		-	-	-	-		-	-		-
50	4	4	-		-	-	-	-		-	-	-	-		-	-		-
100	4	4	-		-	-	-	-		-	-	-	-		-	-		-

Table 2: Visible Symptoms:

Nominal Concentration (mg/l)

Number of fish

symptom (cumulated) after

3hr

6hr

24hr

48hr

72hr

96hr

4

4

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

6.25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

12.5

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

50

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Table 3: pH

		Number of	pH readings after
Nominal	fish		24h		48h		72h		96h
Concentration			V1	V2	V1	V2	V1	V2	V1	V2
(mg/l)
Control	4	4	7.2	7.2	7.5	7.5	7.6	7.6	7.7	7.7
6.25	4	4	7.5	7.5	7.9	7.9	8.0	8.0	8.1	8.1
12.5	4	4	7.5	7.5	7.9	7.9	8.0	8.0	8.1	8.1
25	4	4	7.5	7.5	7.9	7.9	8.1	8.1	8.2	8.2
50	4	4	7.6	7.6	8.0	8.0	8.1	8.1	8.2	8.2
100	4	4	7.6	7.6	8.0	8.0	8.1	8.1	8.2	8.2

Table 4: Test temperature and oxygen content:

Nominal	Number of		Oxygen content (mg/L) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	6.9	6.9	6.8	6.8	6.5	6.5	6.4	6.4
6.25	4	4	6.7	6.7	6.6	6.6	6.4	6.4	6.3	6.3
12.5	4	4	6.7	6.7	6.5	6.5	6.4	6.4	6.3	6.3
25	4	4	6.7	6.7	6.5	6.5	6.4	6.4	6.3	6.3
50	4	4	6.6	6.6	6.5	6.5	6.4	6.4	6.3	6.3
100	4	4	6.6	6.6	6.5	6.5	6.4	6.4	6.3	6.3
Test temperature
Nominal	Number of		Temperature (°C) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	22.9	22.9	23.1	23.1	23.5	23.5	23.3	23.3
6.25	4	4	22.9	22.9	23.1	23.1	23.5	23.5	23.3	23.3
12.5	4	4	22.9	22.9	23.1	23.1	23.5	23.5	23.3	23.3
25	4	4	22.9	22.9	23.1	23.1	23.5	23.5	23.3	23.3
50	4	4	22.9	22.9	23.1	23.1	23.5	23.5	23.3	23.3
100	4	4	22.9	22.9	23.1	23.1	23.5	23.5	23.3	23.3

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical on Zebra fish (Danio rerio) was determine to be > 100 mg/l. As no effect were observed at the higher concentration i.e. 100 mg/l.

Executive summary:

Aim of this study was to evaluate the effect of test chemical test chemical on the mortality rate of Zebra fish (Danio rerio). Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used. Fishes were exposed to these concentrations for 96 hours. The Stability of Test substance was determined as per the Spectrophotometric method and found to be stable for 4 days at 25°C. Plastic aquaria having the water capacity of 5 liters filled with 2 liters of water and were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203 thus consider to be valid. Also fulfills all the necessary criteria. The mortality in the control was found to be 0 per cent at the end of the test. Constant conditions were maintained as far as possible throughout the test and, static procedures used. The dissolved oxygen concentration was found to be 79.76 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item, LC50 was determine to be > 100 mg/l. As no effect (LC0) were observed at the limit concentration of 100 mg/l. Based on the LC50, it can be consider that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Aim of this study was to evaluate the effect of test chemical test chemical on the mortality rate of Zebra fish (Danio rerio). Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used. Fishes were exposed to these concentrations for 96 hours. The Stability of Test substance was determined as per the Spectrophotometric method and found to be stable for 4 days at 25°C. Plastic aquaria having the water capacity of 5 liters filled with 2 liters of water and were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203 thus consider to be valid. Also fulfills all the necessary criteria. The mortality in the control was found to be 0 per cent at the end of the test. Constant conditions were maintained as far as possible throughout the test and, static procedures used. The dissolved oxygen concentration was found to be 79.76 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item, LC50 was determine to be > 100 mg/l. As no effect (LC0) were observed at the limit concentration of 100 mg/l. Based on the LC50, it can be consider that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Based on the various experimental data for the target chemical study have been reviewed to determine the toxic nature of test chemical on the mortality of fish. The studies are as mentioned below:  

Aim of this study was to evaluate the effect of test chemical test chemical on the mortality rate of Zebra fish (Danio rerio). Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous one hour stirring for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25 mg/L,50 mg/L,100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used. Fishes were exposed to these concentrations for 96 hours. The Stability of Test substance was determined as per the Spectrophotometric method and found to be stable for 4 days at 25°C. Plastic aquaria having the water capacity of 5 liters filled with 2 liters of water and were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203 thus consider to be valid. Also fulfills all the necessary criteria. The mortality in the control was found to be 0 per cent at the end of the test. Constant conditions were maintained as far as possible throughout the test and, static procedures used. The dissolved oxygen concentration was found to be 79.76 per cent of the air saturation value throughout the test. After 96 hours of exposure to test item, LC50 was determine to be > 100 mg/l. As no effect (LC0) were observed at the limit concentration of 100 mg/l. Based on the LC50, it can be consider that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

 

First study was supported by the second experimental study for target chemical. Study was conducted to access the effect of test chemical on the mortality rate of Guppy Fish (Poecilia reticulata). Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring for achieving the test concentration of100 mg/L, respectively. Deionized water was used. A limit test performed at 100 mg/l and Guppy Fish (Poecilia reticulata) were exposed to these concentration for 96 hours. Glass bowl aquaria 12” each were filled with 10 liters of water and were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal concentration 100 mg/l, LC50 was determine to be > 100 mg/l. As no effect (LC0) were observed at the limit concentration of 100 mg/l. Based on the LC50, it can be consider that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Based on the above studies, it was conclude that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.