Tetrabutylammonium bromide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

Standard Microtox M500 Rapid Acute Toxicity test:-
The Microtox Acute Toxicity test is an ASTM standard test method that provides a rapid means of determining the acute toxicity of aqueous
compounds by measuring decreases in light output from the luminescent bacterium Vibrio fischeri NRRL B-11177. A decrease in luminescence is
linked to a decrease in respiration, and serves as an indirect measure of the toxicity of the test compound. The Microtox Acute Toxicity test is used
widely for determining the toxicity of single compounds, for monitoring industrial effluents in environmental water quality surveys, and in sediment contamination studies.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

test substance diluted in sterile distilled water.

Test organisms (species):

Vibrio fisheri

Details on inoculum:

luminescent bacterium Vibrio fischeri NRRL B-11177

Test type:

not specified

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

15 min

Nominal and measured concentrations:

The substnace was diluted to 10000 mg/L in sterile distilled water.

Reference substance (positive control):

yes

Key result

Duration:

15 min

Dose descriptor:

EC50

Effect conc.:

1.862 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

growth inhibition

Remarks on result:

other: Log EC50: 0.27+/- 0.07 mg/L

Results with reference substance (positive control):

The quaternary ammonium compounds (including test substance) examined in this study were less toxic to V. fischeri than the pyridinium and imidazolium compounds tested.

Validity criteria fulfilled:

not specified

Conclusions:

Toxicity value of test chemical to luminescent bacterium Vibrio fischeri NRRL B-11177 observed was EC50 (15 minutes) was 1.862 mg/L. The toxic effect depeicted by tetrabutyl ammonium bromide was much less toxic than the pyridinium and imidazolium compounds tested.

Executive summary:

Toxicity value of test chemical to luminescent bacterium Vibrio fischeri NRRL B-11177 observed was EC50 (15 minutes) was observed to be 1.862 mg/L. The toxic effect depeicted by test chemical was much less toxic than the pyridinium and imidazolium compounds tested.

Description of key information

Toxicity value of test chemical to luminescent bacterium Vibrio fischeri NRRL B-11177 observed was EC50 (15 minutes) was observed to be 1.862 mg/L. The toxic effect depeicted by test chemical was much less toxic than the pyridinium and imidazolium compounds tested.

Key value for chemical safety assessment

EC50 for microorganisms:

1.862 mg/L

Additional information

Summarized result for the toxicity of test chemical on the mortality rate of microorganisms.

In the first study from peer reviewed journal toxicity was determine on the bacteria vibrio fischeri. Toxicity value of test chemical to luminescent bacterium Vibrio fischeri NRRL B-11177 observed was EC50 (15 minutes) was observed to be 1.862 mg/L. The toxic effect depicted by test chemical was much less toxic than the pyridinium and imidazolium compounds tested.

 

Above study was supported by the second study from peer reviewed journal. The anti-fungal activity of test chemical was evaluated against two wood decay fungi. 21 day MIC (minimum inhibition concentration) was determine to be >4.2 micro mol/cm2. Tetrabutyl ammonium bromide did not inhibit fungi (Perenniporia tephropora) growth under bioassay conditions.