Tris(methylphenyl) phosphate

Genetic toxicity: in vivo

Administrative data

Endpoint:

genetic toxicity in vivo

Remarks:

Type of genotoxicity: genome mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

1984, not specified.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

GLP. Reported under EC 793/93 for Existing substances regulations. Full methodology etc not available. Deemed reliable as evaluated by the European Commission. This data is considered the property of the data submitter. However it has not been possible to locate the study report due to re-organisation associated with the merger. Read across to structural analogue.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Young adult male flies were treated with the test substance in water-sucrose medium for 3 days. They were then mated with "Basc" females for 3 days so that only late spermatids and spermatozoa were sampled. The F1 brood was grown and mated and the F2 were examined for the presence (non-lethal) or absence (lethal) of wild-type males.

GLP compliance:

yes

Type of assay:

rodent dominant lethal assay

Test material

Test material information

Constituent 1

Reference substance name:

Reofos 50

IUPAC Name:

Reofos 50

Constituent 2

Reference substance name:

Phenol, isopropylated, phosphate (3:1)

EC Number:

273-066-3

EC Name:

Phenol, isopropylated, phosphate (3:1)

Cas Number:

68937-41-7

Molecular formula:

CXHYO4P X and Y are variable dependant on the molecular component.

IUPAC Name:

Phenol, isopropylated, phosphate (3:1)

Test material form:

not specified

Details on test material:

- Name of test material (as cited in study report): Reofos 50
- Molecular formula (if other than submission substance): not applicable
- Molecular weight (if other than submission substance): not applicable
- Smiles notation (if other than submission substance): not applicable
- InChl (if other than submission substance): not applicable
- Structural formula attached as image file (if other than submission substance): not applicable
- Substance type: not specified
- Physical state: not specified
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: not specified
- Isomers composition: not specified
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Radiochemical purity (if radiolabelling): not applicable
- Specific activity (if radiolabelling): not applicable
- Locations of the label (if radiolabelling): not applicable
- Expiration date of radiochemical substance (if radiolabelling): not applicable
- Stability under test conditions: not specified
- Storage condition of test material: not specifed
- Other:

Test animals

Species:

Drosophila melanogaster

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

Young adult male flies were treated with the test substance in water-sucrose medium for 3 days. They were then mated with "Basc" females for 3 days so that only late spermatids and spermatozoa were sampled. The F1 brood was grown and mated and the F2 were examined for the presence (non-lethal) or absence (lethal) of wild-type males.

Administration / exposure

Route of administration:

oral: feed

Vehicle:

water-sucrose medium

Details on exposure:

Young adult male flies were treated with the test substance in water-sucrose medium for 3 days.

Duration of treatment / exposure:

Young adult male flies were treated with the test substance in water-sucrose medium for 3 days.

Frequency of treatment:

Ad libitum.

Post exposure period:

Not specified.

Doses / concentrations

Doses / concentrations 1

Remarks:

Doses / Concentrations:
32.5 mg/ml
Basis:
nominal in diet

Doses / concentrations 2

Remarks:

Doses / Concentrations:
75 mg/ml
Basis:
nominal in diet

Doses / concentrations 3

Remarks:

Doses / Concentrations:
150 mg/ml
Basis:
nominal in diet

No. of animals per sex per dose:

Not specified.

Control animals:

no

Positive control(s):

Not specified; presumed non.

Examinations

Tissues and cell types examined:

Mature germ cells.

Details of tissue and slide preparation:

Not specified.

Evaluation criteria:

Not specified.

Statistics:

Not specified.

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

not specified

Negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

The material tested did not induce mutations in the mature germ cells of Drosophila melanogaster males when administered by feeding

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative
Read across to structural analogue. The material tested did not induce mutations in the mature germ cells of Drosophila melanogaster males when administered by feeding

Executive summary:

Read across to structural analogue. The material tested did not induce mutations in the mature germ cells of Drosophila melanogaster males when administered by feeding. No classification is applicable.