Ethanol

Toxicity to terrestrial arthropods

Administrative data

Endpoint:

toxicity to terrestrial arthropods, other

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Data source

Reference

Reference Type:

publication

Title:

Embryo-larval exposure to atrazine reduces viability and alters oxidative stress parameters in Drosophila melanogaster.

Author:

Figueira FH, de Aguiar LM, da Rosa CE.

Year:

2017

Bibliographic source:

Comparative Biochemistry and Physiology, Part C 191 (2017) 78–85

Materials and methods

Principles of method if other than guideline:

Study to assess the effects of a herbicide on the survival and redox metabolism in the fruit fly D. melanogaster exposed during embryonic and larval development. Ethanol was used as an inert solvent.

GLP compliance:

not specified

Application method:

contact

Test material

Test material information

Constituent 1

Reference substance name:

Ethanol

EC Number:

200-578-6

EC Name:

Ethanol

Cas Number:

64-17-5

Molecular formula:

C2H6O

IUPAC Name:

ethanol

Test material form:

liquid

Test substrate

Vehicle:

no

Test organisms

Test organisms (species):

other: Drosophila Melanogaster

Animal group:

Diptera (fruit fly (small))

Details on test organisms:

D. melanogaster wild type strains were provided by the Santa Maria Federal University – Brazil and maintained in the Terrestrial Animal Room of the Federal University of Rio Grande – Brazil. The flies were maintained at 25 °C under a light/dark cycle of 12 h in conventional medium.

Study design

Study type:

laboratory study

Limit test:

yes

Total exposure duration:

10 d

Remarks:

The development time was evaluated by counting days until adult fly emergence with observations every 24 hours. 10 day was for control.

Test conditions

Details on test conditions:

The parental generation of flies was allowed to lay eggs into Petri dishes with medium. After 24 h, the flies were removed, the embryos collected and transferred to vials with 30 ml of the experimental medium. 30 embryos were used in the ethanol test.

Nominal and measured concentrations:

0.02%

Results and discussion

Effect concentrations

Duration:

10 d

Dose descriptor:

EC0

Effect conc.:

> 0.02 other: %

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: significant difference in % pupation and emergence rate compared to control and sex ratio.

Applicant's summary and conclusion

Executive summary:

In a study to examine the effect of a herbide on the embryonic development the fruit fly Drosophila melanogaster, ethanol was used as an inert solvent and showed no effect on the normal 10 day time for pupation, emergence or sex ratio up to a concentration of 0.02% (200mg/L) in water.