[No public or meaningful name is available]

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Gelb LD 6259
- Lot/batch No.: 0817VP01
- Analytical purity: 87.6 - 89.1
- Physical state: solid

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test was dissolved in distilled water and subsequently homogenised using an ultra turrax

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: fish were hatched at the facility
- Age at study initiation (mean and range, SD): approx. 6 month
- Length at study initiation (length definition, mean, range and SD): 3.3 cm (3.1 - 3.5 cm)
- Weight at study initiation (mean and range, SD): 0.3 g (0.27 - 0.34 g)
- Feeding during test
- Food type: During the last day before start of exposure


ACCLIMATION
- Type and amount of food: Tetramin, TetraWerke, Melle, Germany; ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): < 5 % during last week of acclimatisation

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

1 mmol/l

Test temperature:

22 °C

pH:

8.3 - 8.4

Dissolved oxygen:

8.4 - 8.5 mg/l

Nominal and measured concentrations:

Nominal test concentration: 120 mg/l
The analytically determined concentration was within 20 % of the nominal concentration (115.1 - 115.8 mg/l).

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel:7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.21 g fish /l


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: non chlorinated charcoal filtered tap water mixed with dionised water and aerated
- Conductivity: 250 µS/cm



OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 70 - 570 LUX


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 6, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: LC50 (96 h) > 100 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In conclusion the 96h LC50 for Gelb LD 6259 with the zebra fish (Danio rerio) were >120 mg/L based on the nominal concentration of the test substance and >116 mg/L based on the mean of analytically determined concentrations.