1-amino-4-hydroxy-2-phenoxyanthraquinone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

July 30th, 1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Basic details are provided in the report.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

The test has been conducted according to the internal method A-11.
In principle, the methods described in OECD guideline 203 were used. The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Trout
- Source: P. Hohler, 4314 Zeiningen
- Age at study initiation: 4 - 6 months
- Length at study initiation: 4 - 8 cm
- Weight at study initiation: 3 - 6 g
- Food type: TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany

ACCLIMATION
- Acclimation period: 12 days minimum at 10 - 13 °C
- Feeding frequency: daily

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

96 h

Test temperature:

15 °C

pH:

7.8 - 8.0

Dissolved oxygen:

4.8 mg/l minimum

Nominal and measured concentrations:

Concentrations: 100 mg/l, 300 mg/l and 1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Full glass basins, 17 litres (36 cm × 23 cm × 26 cm)
- Volume of water: 15 litres
- Replicates: 8 per test concentration

TEST MEDIUM / WATER PARAMETERS
Preparation of artificial testing water:
- Stock solution 1
250 g CaCl2.2 H2O (Merck 2382)
29 g NaCl (Merck 6404E)
9 g NaNO3 (Merck 6537)
1 litre of deionized water is added

- Stock solution 2
115 g MgSO4.7H2O (Merck 5886)
79 g Na2SO4 (Merck 822286)
1 litre of deionized water is added

- Stock solution 3
27 g NaHCO3 (Merck 6329E)
1 litre of deionized water is added

- The test water was prepared from the above stock solutions as follows:
7.5 ml Stock solution 1
7.5 ml Stock solution 2
75 ml Stock solution 3
15 litres of deionized water

OTHER TEST CONDITIONS
- Photoperiod: 12 h/12 h day-night cycles

TEST CONCENTRATIONS
- Preliminary study: yes, at 100 mg/l
- Test concentrations: 100 mg/l, 300 mg/l and 1000 mg/l
- Results used to determine the conditions for the definitive study: Result of the preliminary study which showed the LC 50 (96 h) to be greater than 100 mg/l.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

ca. 300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 485 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

< 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 162 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality

No mortality occurred at 100 mg/l (preliminary test group) and at 300 mg/l during the test.

In the 1000 mg/l test group, 1 mortality occurred after 24 h and 7 further mortalities occurred at 48 h.

pH

The following were pH values at each test concentrations after 96 hours:

At 100 mg/l: 7.8

At 300 mg/l: 8.0

At 1000 mg/l: 8.0

Oxygen concentration

The following were oxygen concentrations at each test concentrations after 96 hours:

At 100 mg/l: 8.2

At 300 mg/l: 8.5

At 1000 mg/l: 8.7

Validity criteria fulfilled:

not specified

Conclusions:

LC50 (96 h) = ca. 485 mg/l, equivalent to LC50 = ca. 162 mg/l of active ingredient.

Executive summary:

The substance was tested for short term toxic potential to fish according to an internal method A. 11, comparable to OECD Guideline 203, "Fish, Acute Toxicity Test" adopted on July 17th, 1992.

Test concentrations were 100 mg/l, 300 mg/l and 1000 mg/l. Replicates of 8 Salmo gairdneri (trout) per test concentrations were used. The duration of the test was 96 hours. Mortality of fish was checked at 24 h, 48 h, 72 h and 96 h.

No mortality occurred at 100 mg/l (preliminary test group) and at 300 mg/l during the test. In the 1000 mg/l test group, 1 mortality occurred after 24 h and 7 further mortalities occurred at 48 h.

The LC50 (96 h) of the test substance was calculated to be about 485 mg/l, equivalent to 162 mg/l of active ingredient, given a 33.3% purity of test material.

Therefore, the test substance is nontoxic to fish.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

June 23rd, 1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Basic details are provided in the report.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

The test has been conducted according to the internal method A-11.
In principle, the methods described in OECD Guideline 203 were used. The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Trout
- Source: P. Hohler, 4314 Zeiningen
- Age at study initiation: 4 - 6 months
- Length at study initiation: 4 - 8 cm
- Weight at study initiation: 3 - 6 g
- Food type: TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany

ACCLIMATION
- Acclimation period: 12 days minimum at 10 - 13 °C
- Feeding frequency: daily

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

96 h

Test temperature:

15 °C

pH:

7.8 - 8.0

Dissolved oxygen:

4.8 mg/l minimum

Nominal and measured concentrations:

Concentrations: 100 mg/l, 300 mg/l and 1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Full glass basins, 17 litres (36 cm × 23 cm × 26 cm)
- Volume of water: 15 litres
- Replicates: 8 per test concentration

TEST MEDIUM / WATER PARAMETERS
Preparation of artificial testing water:
- Stock solution 1
250 g CaCl2.2 H2O (Merck 2382)
29 g NaCl (Merck 6404E)
9 g NaNO3 (Merck 6537)
1 litre of deionized water is added

- Stock solution 2
115 g MgSO4.7H2O (Merck 5886)
79 g Na2SO4 (Merck 822286)
1 litre of deionized water is added

- Stock solution 3
27 g NaHCO3 (Merck 6329E)
1 litre of deionized water is added

- The test water was prepared from the above stock solutions as follows:
7.5 ml Stock solution 1
7.5 ml Stock solution 2
75 ml Stock solution 3
15 litres of deionized water

OTHER TEST CONDITIONS
- Photoperiod: 12 h/12 h day-night cycles

TEST CONCENTRATIONS
- Preliminary study: yes, at 100 mg/l
- Test concentrations: 100 mg/l, 300 mg/l and 1000 mg/l
- Results used to determine the conditions for the definitive study: Result of the preliminary study which showed the LC 50 (96 h) to be greater than 100 mg/l

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

ca. 300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 550 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 183 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality

No mortality occurred at 100 mg/l (preliminary test group) and at 300 mg/l during the test.

In the 1000 mg/l test group, 1 mortality occurred after 24 h and 7 further mortalities occurred at 48 h.

pH

The following were pH values at each test concentration after 96 hours:

100 mg/l: 7.8

300 mg/l: 8.0

1000 mg/l: 8.0

Oxygen concentration

The following were oxygen concentrations at each test concentration after 96 hours:

100 mg/l: 8.2 mg/l

300 mg/l: 8.5 mg/l

1000 mg/l: 8.7 mg/l

Validity criteria fulfilled:

not specified

Conclusions:

LC50 (96 h) = ca. 550 mg/l, equivalent to ca. 183 mg/l of active ingredient.

Executive summary:

The substance was tested for short term toxic potential to fish according to an internal method A. 11, comparable to OECD Guideline 203, "Fish, Acute Toxicity Test" adopted on July 17th, 1992.

Test concentrations were 100 mg/l, 300 mg/l and 1000 mg/l. Replicates of 8 Salmo gairdneri (trout) per test concentrations were used. The duration of the test was 96 hours. Mortality of fish was checked at 24 h, 48 h, 72 h and 96 h.

No mortality occurred at 100 mg/l (preliminary test group) and at 300 mg/l during the test. In the 1000 mg/l test group, 1 mortality occurred after 24 h and 7 further mortalities occurred at 48 h. The LC50 (96 h) of the test substance was interpolated to be about 550 mg/l, equivalent to 183 mg/l of active ingradient given a 33.3 % purity of test material.

Therefore, the test substance is nontoxic to fish.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

February 24th, 1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Basic details are provided in the report.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

The test has been conducted according to the internal method A-11.
In principle, the methods described in OECD Guideline 203 were used. The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Trout
- Source: P. Hohler, 4314 Zeiningen
- Age at study initiation: 4 - 6 months
- Length at study initiation: 4 - 8 cm
- Weight at study initiation: 3 - 6 g
- Food type: TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany

ACCLIMATION
- Acclimation period: 12 days minimum at 10 - 13 °C
- Feeding frequency: daily

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 h

Test temperature:

15 °C

pH:

7.7 - 7.9

Dissolved oxygen:

4.8 mg/l minimum

Nominal and measured concentrations:

Concentrations: 100 mg/l, 200 mg/l and 1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Full glass basins, 17 litres (36 cm × 23 cm × 26 cm)
- Volume of water: 15 litres
- Replicates: 8 per test concentration

TEST MEDIUM / WATER PARAMETERS
Preparation of artificial testing water:
- Stock solution 1
250 g CaCl2.2 H2O (Merck 2382)
29 g NaCl (Merck 6404E)
9 g NaNO3 (Merck 6537)
1 litre of deionized water is added

- Stock solution 2
115 g MgSO4.7H2O (Merck 5886)
79 g Na2SO4 (Merck 822286)
1 litre of deionized water is added

- Stock solution 3
27 g NaHCO3 (Merck 6329E)
1 litre of deionized water is added

- The test water was prepared from the above stock solutions as follows:
7.5 ml Stock solution 1
7.5 ml Stock solution 2
75 ml Stock solution 3
15 litres of deionized water

OTHER TEST CONDITIONS
- Photoperiod: 12 h/12 h day-night cycles

TEST CONCENTRATIONS
- Preliminary study: yes, at 100 mg/l
- Test concentrations: 100 mg/l, 200 mg/l and 1000 mg/l
- Results used to determine the conditions for the definitive study: Result of the preliminary study

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

ca. 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 580 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

ca. 193 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality

No mortality occurred at 200 mg/l and 100 mg/l.

Two mortalities occurred at 1000 mg/l after 48 h.

Validity criteria fulfilled:

not specified

Conclusions:

LC50 (48 h) = ca. 580 mg/l, equivalent to ca. 183 mg/l of active ingredient.

Executive summary:

The substance was tested for short term toxic potential to fish according to an internal method A. 11, comparable to OECD Guideline 203, "Fish, Acute Toxicity Test" adopted on July 17th, 1992.

Test concentrations were 100 mg/l, 200 mg/l and 1000 mg/l. Replicates of 8 Salmo gairdneri (trout) per test concentrations were used. The duration of the test was 48 hours. Mortality of fish was checked at 24 h and 48 h.

No mortality occurred at 100 mg/l and 200 mg/l during the test. In the 1000 mg/l group, no mortality occurred at 24 h and 6 mortalities occurred at 48 h.

The LC 50 (48 h) of the test substance was determined to be about 580 mg/l, equivalent to 183 mg/l of active ingredient given a 33.3 % purity of test material.

Therefore, the test substance is nontoxic to fish.

Reference 4

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

From July 4th to 30th, 1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Basic details are provided in the report.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

The test has been conducted according to the internal method A-11.
In principle, the methods described in OECD Guideline 203 were used. The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium GmbH, D-3422 Bad Lauterberg
- Age at study initiation: 4 - 6 months
- Length at study initiation: about 3 cm
- Weight at study initiation: 0.25 - 0.35 g

ACCLIMATION
- Acclimation period: 12 days minimum at 10 - 13 °C
- Feeding frequency: daily

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23 °C

pH:

8.2

Dissolved oxygen:

4.8 mg/l minimum

Nominal and measured concentrations:

Concentrations: 300 mg/l and 1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Full glass basins, 17 litres (36 cm × 23 cm × 26 cm)
- Volume of water: 3 litres
- Replicates: 10 per test concentration

TEST MEDIUM / WATER PARAMETERS
Preparation of artificial testing water:
- Stock solution 1
250 g CaCl2.2 H2O (Merck 2382)
29 g NaCl (Merck 6404E)
9 g NaNO3 (Merck 6537)
1 litre of deionized water is added

- Stock solution 2
115 g MgSO4.7H2O (Merck 5886)
79 g Na2SO4 (Merck 822286)
1 litre of deionized water is added

- Stock solution 3
27 g NaHCO3 (Merck 6329E)
1 litre of deionized water is added

- The test water was prepared from the above stock solutions as follows:
7.5 ml Stock solution 1
7.5 ml Stock solution 2
75 ml Stock solution 3
15 litres of deionized water

OTHER TEST CONDITIONS
- Photoperiod: 12 h/12 h day-night cycles

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Mortality of fish was checked at 24 h, 48 h, 72 h and 96 h

TEST CONCENTRATIONS
- Preliminary study: yes, at 100 mg/l
- Test concentrations: 300 mg/l and 1000 mg/l
- Results used to determine the conditions for the definitive study: Result of the preliminary study which showed the LC50 (96 h) to be greater than 100 mg/l.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 333 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality

There was no mortality of fish at all test concentrations during the test.

pH

The following were pH values at each test concentration after 96 hours:

At 300 mg/l: 8.2

At 1000 mg/l: 8.2

Oxygen concentration

The following were oxygen concentrations at each test concentration after 96 hours:

At 300 mg/l: 7 mg/l

At 1000 mg/l: 8.2 mg/l

Validity criteria fulfilled:

not specified

Conclusions:

LC50 (96 h) > 1000 mg/l, equivalent to LC50 > 333 mg/l of active ingredient.

Executive summary:

The substance was tested for short term toxic potential to fish according to an internal method A. 11, comparable to OECD Guideline 203, "Fish, Acute Toxicity Test" adopted on July 17th, 1992.

A preliminary study was performed at 100 mg/l. Test concentrations were 300 mg/l and 1000 mg/l. Replicates of 10 fish (Brachydanio rerio) per test concentrations were used. The duration of the test was 96 hours. Mortality of fish was checked at 24 h, 48 h, 72 h and 96 h.

No mortality occurred at all test concentrations during the test. The LC50 (96 h) of the test substance is greater than 1000 mg/l, equivalent to 333 mg/l of active ingredient given a 33 % purity of test material.

Therefore, the test substance is nontoxic to fish.

Reference 5

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

January 15th, 1990

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Remarks:

Only few details on the study are available. Test conditions and test organism details unavailable. Result considered plausible for a risk assessment.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

360 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

The substance was tested for its potential to be acutely toxic to fish according to OECD Test Guideline 203.

However, further data on test organisms and test conditions were not given in the report.

Validity criteria fulfilled:

not specified

Conclusions:

LC50 (96 h) = 360 mg/l.

Executive summary:

The substance was tested for short term toxic potential to fish according to OECD Test Guideline 203, "Fish, Acute Toxicity Test". Brachydanio rerio was the specie tested. The duration of the test was 96 hours. Mortality of fish after 96 h of exposure to the substance was the basis of toxicity evaluation.

The following effect levels of the test substance, in terms of active ingredient, were determined: LC0 = 200 mg/l, LC50 = 360 mg/l and LC100 > 1000 mg/l.

Reference 6

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

April 24th, 1978

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Remarks:

The test was carried out according to a scientifically acceptable method comparable to a guideline study. Substance purity available. Test concentrations and duration unavailable. Result considered plausible for a risk assessment.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

The test has been conducted according to the internal method A-11.
In principle, the methods described in OECD Guideline 203 were used. The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Trout
- Source: P. Hohler, 4314 Zeiningen
- Age at study initiation: 4 - 6 months
- Length at study initiation: 4 - 8 cm
- Weight at study initiation: 3 - 6 g
- Food type: TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany

ACCLIMATION
- Acclimation period: 12 days minimum at 10 - 13 °C
- Feeding frequency: daily

Test type:

static

Water media type:

not specified

Limit test:

no

Test temperature:

15 °C

pH:

6 - 8

Dissolved oxygen:

4.8 mg/l minimum

Nominal and measured concentrations:

No data

Details on test conditions:

TEST SYSTEM
- Test vessel: Full glass basins, 17 litres (36 cm × 23 cm × 26 cm)
- Volume of water: 15 litres
- Replicates: 8 per test concentration

TEST MEDIUM / WATER PARAMETERS
Preparation of artificial testing water:
- Stock solution 1
250 g CaCl2.2 H2O (Merck 2382)
29 g NaCl (Merck 6404E)
9 g NaNO3 (Merck 6537)
1 litre of deionized water is added

- Stock solution 2
115 g MgSO4.7H2O (Merck 5886)
79 g Na2SO4 (Merck 822286)
1 litre of deionized water is added

- Stock solution 3
27 g NaHCO3 (Merck 6329E)
1 litre of deionized water is added

- The test water was prepared from the above stock solutions as follows:
7.5 ml Stock solution 1
7.5 ml Stock solution 2
75 ml Stock solution 3
15 litres of deionized water

OTHER TEST CONDITIONS
- Photoperiod: 12 h/12 h day-night cycles

Reference substance (positive control):

not specified

Dose descriptor:

LC50

Effect conc.:

580 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: duration not available

Dose descriptor:

LC50

Effect conc.:

230 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Remarks on result:

other: duration not available

Sublethal observations / clinical signs:

Effect levels

The exposure duration was not given in the report. However, the effect levels available are:

LC0 = 200 mg/l

LC50 = 580 mg/l

LC100 > 1000 mg/l

Validity criteria fulfilled:

not specified

Conclusions:

LC50 = ca. 580 mg/l, equivalent to ca. 230 mg/l of active ingredient.

Executive summary:

The substance was tested for short term toxic potential to fish according to an internal method n. 11, comparable to OECD guideline 203 (1992).

Replicates of 8 Salmo gairdneri (trout) per test concentration were used. The duration of the test was not given.

The LC50 of the test substance was determined to be about 580 mg/l, equivalent to 230 mg/l of active ingredient, given a purity of 39.6 % of test material.

Therefore, the test substance is nontoxic to fish.

Description of key information

LC50 (96 h) = ca. 162 mg/l of active ingredient.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

162 mg/L

Additional information

The short-term acute toxicity to fish species was investigated in seven experiments carried out according to scientifically acceptable methods comparable to OECD test guideline 203.

96-hour tests on Brachydanio rerio fish led to LC50 > 333 mg/l and LC50 = 360 mg/l in terms of active ingredient.

Salmo gairdneri fish was also used and the following LC50 values were obtained in 96-hour tests: 550 and 485 mg/l. LC50s obtained in 48-hour tests were: 580 and 130 mg/l. LC50 (duration unavailable) in another test was 580 mg/l. Based on the active ingredient content in test material, these values are all above the threshold limit of classification for aquatic toxicity in the CLP Regulation (EC 1272/2008), namely 100 mg/l, except for LC50 = 130 mg/l (LC50 = 42 mg/l as active ingredient). However, this result was disregarded as not confirmed by other available tests on the same species. In addition, the reliability of this study couldnot be assessed due to the lack of experimental details.

The most restrictive result i.e. LC50 (96 h) = ca. 485 mg/l, equivalent to 162 mg/l of active ingredient, was taken as reference.

The water solubility of the substance is << 0.02 g/l as determined by the flask method in OECD guideline 105 (1) (2). Therefore, under test conditions, concentration of test substance in water is 20 -30 mg/l, while the excess is undissolved in water (1) (2). Despite this undissolved fraction, no signs of physical effects on test organisms were seen.

Therefore, it can be concluded that the substance is non-toxic to fish at the concentrations tested.

(1) ETAD Project E 3020 Ecological Assessment of Important Dyestuffs, Dystar Colours Distribution GmbH, 1990

(2) ANALYSIS REPORT N. RC / 025-2 / 15, REACH&Colours Kft, 2015