Phosphonic acid, P-2-propen-1-yl-, diethyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01 Dec 2014 to 31 Dec 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl allylphosphonate

EC Number:

619-646-5

Cas Number:

1067-87-4

Molecular formula:

C7H15O3P

IUPAC Name:

Diethyl allylphosphonate

Test material form:

other: liquid

Details on test material:

Identification: Diethyl Allylphosphonate
Appearance/Physical State: Clear colorless liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: at 0 and 48 h; in the pre-test at 0, 0.1, 1.0, 10 and 100 mg/L, in the final test at 0, 10, 18, 32, 56 and 100 mg/L (bulk smaples at 0 h; pooled samples at 48 h)
- Sample storage conditions before analysis: at -20 °C

Test solutions

Vehicle:

no

Details on test solutions:

test substance was directly added to the test water (preparation under laboratory safety lighting due to potential light sensitivity of the substance)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation: < 24 hours
- Feeding in culture: algae suspension and tetramin flake food suspension

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/L as CaCO2

Test temperature:

20 °C

pH:

7.7-8.1

Dissolved oxygen:

8.8-9.0 mg/L (97-99% of saturation)

Nominal and measured concentrations:

pre-test: nominal 0, 0.1, 1.0, 10 and 100 mg/L; measured at 10 mg/L 95% and 88% of nominal at 0 and 48 h respectively; at 100 mg/L 93% and 88% of nominal at 0 and 48 h respectively
main test: nominal 0, 10, 18, 32, 56 and 100 mg/L; measured at 0 mg/L < LOQ at 0 and 48 h, at 100 mg/L 94 % and 95% of nominal at 0 and 48 h respectively

Details on test conditions:

TEST SYSTEM
- Test vessel: 150 mL
- Type: covered
- Fill volume: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water (according to guidance)
- Alkalinity: pH 7.8 ± 0.2
- Ca/mg ratio: 4/1
- Culture medium different from test medium: Elendt M7

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16/8
- Light intensity: darkness

EFFECT PARAMETERS MEASURED : immobilization

TEST CONCENTRATIONS
- Range finding study: 0, 0.1, 1.0, 10, 100 mg/L
- Test concentrations: 0, 10, 18, 32, 56 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

In the pre-test at 100 mg/L 3 daphnids were immobilized at 24 h and 4 at 48 h. No immobilization at any of the other concentrations.
Based on these results the main study was performed as a full test.
As the measured concentrations were > 80% of nominal, the results are based on nominal concentrations

Results with reference substance (positive control):

The 48h-EC50 for potassium dichromate was 0.58 mg/L

Reported statistics and error estimates:

NA

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 in daphnia is > 100 mg/L

Executive summary:

Daphnia were exposed to 0, 10, 18, 32, 56 and 100 mg/L test substance during 48 hours. No immobilization was found. The analyses for accuracy of preparations showed that the average measured concentrations were > 80% of nominal. Therefore the results are based on nominal concentrations.The EC50 is > 100 mg/L