4-nitrophenylacetic acid

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study according to international accepted guideline and GLP compliant.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-nitrophenylacetic acid

EC Number:

203-168-5

EC Name:

4-nitrophenylacetic acid

Cas Number:

104-03-0

Molecular formula:

C8H7NO4

IUPAC Name:

4-nitrophenylacetic acid

Test material form:

solid: crystalline

Details on test material:

Test item: 4-nitrophenyl-acetic acid
CAS number: 104-03-0
EC number: 203-168-5
Molecular formula: C8H7NO4
Molecular weight: 181.15 g/mol
Physical state: Solid, crystalline powder
Colour: Yellowish
Storage: Cool, dry place, protected from light

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Test species: Pseudokirchneriella subcapitata (Korshikov) F.Hindák (formerly known as Selenastrum capricornutum)
Source: UTEX The Culture Collcetion of Algae (USA).
The test organism was grown and hold in OECD medium which was used also for preparing of saturated test item solution.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

The temperature inside the incubator at the test vessels was measured and registered continuously by thermometer and datalogger. Throughout the test the temperature was nearly constant.

pH:

The measured mean pH values at the beginning of the test varied in the range of 6.32 - 7.63.
The measured mean pH values at the end of the test varied in the range of 7.87 - 9.44.

Nominal and measured concentrations:

In the test the following nominal concentrations were applied: 60.04; 30.02; 15.01; 10.01; 5.00; 3.00; and 1.50 mg/L.
the geometric mean value of measured
and calculated concentrations of 1.33; 0.92; 0.64; 0.51; 0.35; 0.27 and 0.20 mg test
item/L.

Details on test conditions:

The toxic property of test item was investigated at seven concentrations which were prepared by different dilutions of stock solution of test item. At each concentration level three replicates were used. In the case of control six replicates were applied. The initial alga concentration was 5 000 cells/ml. The vessels were closed with silicone bungs with a filter for ensuring the proper air exchange. The vessels were kept in incubator at 21 ± 2 °C with continuous illumination.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.24 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

0.26 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.32 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

The ECx for specific growth rate and yield were determined by ToxRat® statistic software.
The values of LOEC and NOEC were also calculated by ToxRat® statistic software but the results were not conclusive or determinable.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

According to this Freshwater Alga and Cyanobacteria, Growth Inhibition Test for 4-nitrophenyl-acetic acid (CAS No. 104-03-0) the results indicated that the test item is hazardous to the aquatic environment and classified into the first acute toxic hazardous category [GHS Acute aquatic hazard Category Acute I. – 72 hr EC50 (for algae or other aquatic plants) ≤ 1 mg/L].